A preliminary study on the development of an easy method for beef freshness using a cyclic voltammetric system |
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| Authors: | DH Noh SH Chung SJ Choi SJ Hur |
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| Affiliation: | 1. The Korean Intellectual Property Office, Government Complex Bldg 4, Daejeon 302-701, Republic of Korea;2. Department of Food Science, University of Massachusetts Amherst, 100 Holdsworth Way, Amherst, MA 01003, United States;1. Institute of Petrolem and Commodity, Harbin University of Commerce, Harbin;150076, P R China;2. Department of Applied Chemistry, Harbin Institute of Technology, Harbin 150001, P R China;3. Department of Chemical and Materials Engineering, National Kaohsiung University of Applied Sciences, Kaohsiung 807, Taiwan;1. International Postgraduate Programs in Environmental Management, Graduate School, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand;2. Center of Excellence for Environmental and Hazardous Waste Management (EHWM), Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand;3. Department of Chemical Technology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand;4. Center for Petroleum, Petrochemicals and Advanced Materials, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand;5. Department of Environmental Engineering, Faculty of Engineering, Chulalongkorn University, 254 Phayathai Road, Bangkok 10330, Thailand;1. Dipartimento di Fisica e Chimica, Università di Palermo, Viale delle Scienze, Edificio 18, 90128 Palermo, Italy;2. Gruppo V Sezione INFN, Catania, Italy;3. Dipartimento Energia, Ingegneria dell’Informazione e Modelli Matematici, Università di Palermo, Viale delle Scienze, Ed. 6, I-90128 Palermo, Italy;4. Dipartimento di Chimica, Università di Pavia, V.le Taramelli, 12 Pavia, Italy and INFN, Sezione di Pavia, Pavia, Italy.;1. Laboratório Nacional de Nanotecnologia para o Agronegócio (LNNA), Embrapa Instrumentação, 13560-970, São Carlos, SP, Brazil;2. Instituto de Física de São Carlos, Universidade de São Paulo, 13560-970, São Carlos, SP, Brazil;3. Instituto de Física Gleb Wataghin, Unicamp, 13083-859, Campinas, SP, Brazil |
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| Abstract: | This study was carried out to develop an easy method for beef freshness using a cyclic voltammetric system. Prime graded (USDA) beef loin at 48 h postmortem was cut into pieces 3 cm thick, packaged into commercial plastic zipper bag, and then stored at 4 °C for 0, 3, 6 and 9 days. A commercial cyclic voltammetry and data processing software were used for this study and the interdigitated electrode was fabricated according to semiconductor manufacturing. The homogenated beef was filtered through Whatman No. 1 filter paper and 250 μL of the filtrate was applied on the interdigitated electrode sensor chip for electrochemical detection. Electrochemical signals changed as storage periods increased. The shape of the cyclic voltammogram changed from a roundish curve to a shapely curve. Redox values (V) also increased during storage. Electrochemical signals matched well with lipid oxidation values (Thiobarbituric acid reactive substances: TBARS). A linear relationship between redox value and TBARS was obtained with a correlation factor of 0.957. Thus, the electrochemical signal of a cyclic voltammetric system can be used as an indicator of beef freshness. |
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