Real-time PCR assay for detection of Trichinella in meat |
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| Authors: | Mercedes Alonso Beatriz Herrero Juan M Vieites Montserrat Espiñeira |
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| Affiliation: | 1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 730046 Lanzhou, PR China;2. JRU BIPAR ENVA ANSES UPEC, 94703 Maisons-Alfort Cedex, France;1. Department of Infectious Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy;2. Austrian Agency for Health and Food Safety, Innsbruck, Austria;3. Institute of Tropical Medicine, Antwerp, Belgium;4. Veterinary and Food Laboratory, Tartu, Estonia;5. National Veterinary Institute, Uppsala, Sweden;1. Laboratory of Parasitology, Fish and Bee Diseases, Veterinary Diagnostic Directorate, National Food Chain Safety Office, Tábornok utca 2, H-1143 Budapest, Hungary;2. Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanitá, viale Regina Elena 299, 00161 Rome, Italy;1. Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal;2. Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal;3. Victor Caeiro Laboratory of Parasitology, Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), University of Évora, Portugal;4. Institute of Parasitology, Vetsuisse Faculty and Faculty of Medicine, University of Bern, Switzerland |
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| Abstract: | Trichinella are a group of widely distributed parasites which have acquired high social relevance due to their involvement in foodborne infections caused by consumption of raw or undercooked food. A TaqMan®-LNA probe Real-time PCR assay targeting the 5S rRNA was developed allowing the simultaneous detection of the 10 species and 3 genotypes of Trichinella present in meat tissues. The detection limit employing dilutions of genomic DNA was 2 pg and the determination of the detection limt in terms of ppm was 1 ppm.The main novelty of this work lies in the fact that it can assure the absence of 10 species and 3 genotypes of Trichinella in an only assay. The proposed methodology is rapid, robust, highly sensitive and readily adaptable in routine molecular diagnostic laboratories, and can be employed as molecular screening method in order to assess the food security. |
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