Detection of potentially mycotoxigenic Aspergillus species in Capsicum powder by a highly sensitive PCR-based detection method |
| |
| Authors: | Noelia Sardiñas Jéssica Gil-Serna Liliana Santos Antonio J. Ramos María Teresa González-Jaén Belén Patiño Covadonga Vázquez |
| |
| Affiliation: | 1. Department of Microbiology III, Faculty of Biology, University Complutense of Madrid, José Antonio Novais 2, 28040 Madrid, Spain;2. Food Technology Department, University of Lleida, XaRTA-UTPV, Av. Alcalde Rovira Roure 191, 25198 Lleida, Spain;3. Department of Genetics, Faculty of Biology, University Complutense of Madrid, José Antonio Novais 2, 28040 Madrid, Spain;1. Centre of Competence for the Innovation in the Agro-environmental Sector (AGROINNOVA), University of Turin, Grugliasco, TO, Italy;2. DISAFA, Department of Agricultural, Forestry and Food Sciences, University of Turin, Grugliasco, TO, Italy;1. Laboratory of Microbiology and Biotechnology of Foods, Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece;2. Benaki Phytopathological Institute, Department of Phytopathology, Laboratory of Mycology, St. Delta 8, 14561 Athens, Greece;1. Biology Department, Faculty of Science, King Abdulaziz University, Jeddah (KAU), Saudi Arabia;2. Food Toxicology and Contaminants, National Research Center, Cairo, Egypt;3. Department of Pediatric Hematology/Oncology, Yousef Abdullatif Jameel Chair of Prophetic Medicine Application, King Abdulaziz University Hospital (KAUH), Faculty of Medicine, KAU, Saudi Arabia;4. Special Infectious Agents Unit, King Fahd Medical Research Center, Yousef Abdullatif Jameel Chair of Prophetic Medicine Application, KAU, Saudi Arabia;1. Department of Applied Chemistry, Government College University Faisalabad, 38000, Pakistan;2. Food Toxicology Lab., Nuclear Institute for Agriculture and Biology (NIAB), P.O. Box 128, Faisalabad, 38950, Pakistan;3. Department of Biochemistry, Government College University Faisalabad, 38000, Pakistan;4. Department of Radiology, School of Medicine, New York University, USA |
| |
| Abstract: | The objective of this study was to gain information about the toxigenic Aspergillus species present in a wide survey of retail samples of paprika and chilli collected in Spain. Detection of these mycotoxigenic species was performed with an optimized protocol for paprika and chilli which includes a set of species-specific PCR assays. Occurrence of toxigenic Aspergillus species was higher in paprika than in chilli samples (83.9% and 64.5%, respectively). Paprika showed also the highest percentage of co-occurrence of two or more different species (43.6%) in comparison with chilli (35.5%). The most common aspergilli were Aspergillus niger aggregate (67.7%), followed by Aspergillus flavus (49.5%). Aspergillus carbonarius, Aspergillus parasiticus and Aspergillus steynii were detected at lower frequency (1.1%). The high co-occurrence of Aspergillus species able to produce ochratoxin A and aflatoxins, particularly in paprika, suggested the need of a more efficient control during processing and storage to reduce fungal contamination, and additional legislation to consider the simultaneous presence of both toxins in these matrices. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
