Increasing mouse embryonic fibroblast cells adhesion on superhydrophilic vertically aligned carbon nanotube films |
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| Authors: | AO Lobo FR Marciano SC Ramos MM Machado EJ Corat MAF Corat |
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| Affiliation: | 1. Laboratory of Biomedical Nanotechnology (NanoBio), Instituto de Pesquisa e Desenvolvimento (IP&D), Universidade do Vale do Paraíba (UniVap), Avenida Shishima Hifumi 2911, São José dos Campos, 12244-000, SP, Brazil;2. Laboratory of Biomedical Vibrational Spectroscopy (LEVB), Instituto de Pesquisa e Desenvolvimento (IP&D), Universidade do Vale do Paraíba (UniVap), Avenida Shishima Hifumi 2911, São José dos Campos, 12244-000, SP, Brazil;3. Laboratório Associado de Sensores e Materiais (LAS), Instituto Nacional de Pesquisas Espaciais (INPE), Avenida dos Astronautas 1758, São José dos Campos, 12.245-970, SP, Brazil;4. Centro Multidisciplinar para Investigacão Biológica na Área da Ciência em Animais de Laboratório (CEMIB), Universidade Estadual de Campinas (UNICAMP), Rua 05 de Junho s/n°, Cidade Universitária “Zeferino Vaz”, 13083–877, Campinas, Brazil |
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| Abstract: | We have analyzed the adhesion of mouse embryonic fibroblasts (MEFs) genetically modified by green fluorescence protein (GFP) gene cultured on vertically-aligned carbon nanotubes (VACNTs) after 6 days. The VACNTs films grown on Ti were obtained by microwave plasma chemical vapor deposition process using Fe catalyst and submitted to an oxygen plasma treatment, for 2 min, at 400 V and 80 mTorr, to convert them to superhydrophilic. Cellular adhesion and morphology were analyzed by scanning electron, fluorescence microscopy, and thermodynamics analysis. Characterizations of superhydrophilic VACNTs films were evaluated by contact angle and X-Ray Photoelectron Spectroscopy. Differences of crowd adhered cells, as well as their spreading on superhydrophilic VACNTs scaffolds, were evaluated using focal adhesion analysis. This study was the first to demonstrate, in real time, that the wettability of VACNTs scaffolds might have enhanced and differential adherence patterns to the MEF-GFP on VACNTs substrates. |
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