| 两种检测糖化血红蛋白(HbA1c)方法的比较 |
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| 引用本文： | 李俊敏,杨莹,董旭婉,李杉,王菊芳,曹成喜.两种检测糖化血红蛋白(HbA1c)方法的比较[J].现代食品科技,2016,32(1):284-289. |
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| 作者姓名： | 李俊敏 杨莹 董旭婉 李杉 王菊芳 曹成喜 |
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| 作者单位： | （1.华南理工大学生物科学与工程学院，广东省发酵与酶工程重点实验室，广东广州 510006）,（1.华南理工大学生物科学与工程学院，广东省发酵与酶工程重点实验室，广东广州 510006）,（1.华南理工大学生物科学与工程学院，广东省发酵与酶工程重点实验室，广东广州 510006）,（1.华南理工大学生物科学与工程学院，广东省发酵与酶工程重点实验室，广东广州 510006）,（1.华南理工大学生物科学与工程学院，广东省发酵与酶工程重点实验室，广东广州 510006）,（2.上海交通大学生命科学与技术学院，上海 200240） |
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| 基金项目： | 国家重大科学仪器设备开发专项项目“双向凝胶电泳（2DE）成套设备技术开发与应用的研究”（2011YQ030139） |
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| 摘 要： | 血液中糖化血红蛋白(Hb A1c)水平是衡量人体内长期血糖状态的标准。国际临床化学和实验医学联合会(IFCC)将高效液相离子交换层析(HPLC)作为检测糖化血红蛋白的标准参考方法;而20 mmol/L Na OH(阴极电解液)和20 mmol/L H2SO4(阳极电解液)形成的稳定p H梯度及含两性电解质的凝胶使毛细管等电聚焦电泳(c IEF)在窄PH梯度下对糖化血红蛋白有着显著的分离效率;本文将对HPLC与凝胶c IEF进行分析比较。采用HPLC和凝胶c IEF方法分别测定随机46例血样样品的Hb A1c值,对两组测定结果进行相关性分析。表明两种测定方法相关性良好;但是,由于HPLC方法易受血红蛋白变异体干扰,特异性较低;c IEF不受血红蛋白变异体干扰,特异性高。因此,凝胶c IEF方法可以作为HPLC的替代方法应于糖化血红蛋白的标准检测
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| 关 键 词： | 糖化血红蛋白 高效液相色谱 毛细管等电聚焦 |
| 收稿时间： | 2015/4/25 0:00:00 |
Comparison of Two Methods for Glycated Hemoglobin (HbA1c) Measurement |
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| LI Jun-min,YANG Ying,DONG Xu-wan,LI Shan,WANG Ju-fang and CAO Cheng-xi.Comparison of Two Methods for Glycated Hemoglobin (HbA1c) Measurement[J].Modern Food Science & Technology,2016,32(1):284-289. |
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| Authors: | LI Jun-min YANG Ying DONG Xu-wan LI Shan WANG Ju-fang and CAO Cheng-xi |
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| Affiliation: | (1.School of Bioscience and Bioengineering, South China University of Technology, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Guangzhou 510006, China),(1.School of Bioscience and Bioengineering, South China University of Technology, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Guangzhou 510006, China),(1.School of Bioscience and Bioengineering, South China University of Technology, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Guangzhou 510006, China),(1.School of Bioscience and Bioengineering, South China University of Technology, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Guangzhou 510006, China),(1.School of Bioscience and Bioengineering, South China University of Technology, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, Guangzhou 510006, China) and (2.Life Science and Technology, Shanghai Jiaotong University, ShangHai 200240, China) |
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| Abstract: | The level of glycated hemoglobin (HbA1c) in the blood is a standard measure of the long-term glycemic status of the human body. High-performance liquid chromatography (HPLC) is the standard reference method for HbA1c measurement as designated by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). Gel-capillary isoelectric focusing (cIEF) with a stable pH gradient of 20 mmol/L NaOH (catholyte solution) and 20 mmol/L sulfuric acid (anolyte solution) and gels containing ampholytes have outstanding separation power. In this study, HPLC has been compared to gel-cIEF for the measurement of HbA1c. HPLC and gel-cIEF were used to measure the HbA1c values of 46 randomly selected blood samples. Correlation analysis revealed that the two methods showed a good correlation. The HPLC method was prone to interference by hemoglobin variants and exhibited low specificity. The gel-cIEF method was not affected by hemoglobin variants and showed high specificity. In conclusion, gel-cIEF can be used as an alternative method to HPLC for the standard detection of glycated hemoglobin. |
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| Keywords: | glycated hemoglobin high performance liquid chromatography capillary isoelectric focusing |
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