| 高效液相色谱法定量分析竹节参中人参皂苷Rb1的含量 |
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| 引用本文： | 刘少静,杨黎彬,王小宁,康辛莉. 高效液相色谱法定量分析竹节参中人参皂苷Rb1的含量[J]. 应用化工, 2012, 41(6): 1089-1091 |
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| 作者姓名： | 刘少静 杨黎彬 王小宁 康辛莉 |
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| 作者单位： | 西安医学院药学院,陕西西安,710021 |
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| 摘 要： | 用RP-HPLC测定竹节参中人参皂苷Rb1的含量,采用Venusil ABS C18色谱柱(250 mm×4.6 mm5,μm),以乙腈-水(35∶65)为流动相,流速1 mL/min,检测波长203 nm。结果表明,人参皂苷Rb1在25～500μg/mL间线性关系良好(r=0.999 4),平均加样回收率为99.64%,RSD=1.52%。该方法快速、准确、重现性好,可用于竹节参中人参皂苷Rb1的含量测定。
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| 关 键 词： | 高效液相色谱法 竹节参 人参皂苷Rb1 含量测定 |
Determination of ginsenoside Rb1 in Panax japonicus C.A.Meyer by HPLC |
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| LIU Shao-jing , YANG Li-bin , WANG Xiao-ning , KANG Xin-li. Determination of ginsenoside Rb1 in Panax japonicus C.A.Meyer by HPLC[J]. Applied chemical industry, 2012, 41(6): 1089-1091 |
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| Authors: | LIU Shao-jing YANG Li-bin WANG Xiao-ning KANG Xin-li |
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| Affiliation: | (College of Pharmacy,Xi’an Medical University,Xi’an 710021,China) |
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| Abstract: | Determination of the content of ginsenoside Rb1 in Panax japonicus C.A.Meyer.The analytical column was Venusil ABS C18(250 mm× 4.6 mm,5 μm).The mobile phase consisted of acetonitrile-water(35 ∶ 65).The flow rate was 1.0 mL/min and the detection wavelength was 203 nm.Results showed that the linear range of ginsenoside Rb1 was at 25～500 μg/mL(r=0.999 4),and the average recovery was 99.64%,RSD was 1.52%.The method is quick,accurate,good reproducibility and can be used for the content determination of ginsenoside Rb1 in Panax japonicus C.A.Meyer. |
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| Keywords: | HPLC Panax japonicus C.A.Meyer ginsenoside Rb1 content determination |
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