| 大孔树脂分离纯化沙芥黄酮的工艺研究 |
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| 引用本文： | 温俊峰,刘步明.大孔树脂分离纯化沙芥黄酮的工艺研究[J].应用化工,2012,41(5):827-830. |
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| 作者姓名： | 温俊峰 刘步明 |
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| 作者单位： | 榆林学院化学与化工学院,陕西榆林,719000 |
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| 基金项目： | 榆林市科技计划项目，榆林学院院级科研项目 |
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| 摘 要： | 用大孔吸附树脂分离纯化沙芥总黄酮,比较了7种大孔树脂对沙芥总黄酮的静态吸附动力学特性,优选出D-4020型大孔吸附树脂分离纯化沙芥总黄酮,并对其进行动态吸附实验。结果表明,D-4020纯化沙芥总黄酮的最佳工艺参数为:上样液浓度0.4 mg/mL,pH值5,上样流速2 mL/min;使用4BV用量95%的乙醇作为洗脱剂,洗脱流速为2 mL/min。采用该工艺分离纯化沙芥总黄酮含量达40.91%。
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| 关 键 词： | 沙芥 总黄酮 分离纯化 大孔树脂 |
Purification of flavonoids extracted from Pugionium cornutum (L.) Gaerth.by macroporous resins |
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| WEN Jun-feng
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LIU Bu-ming.Purification of flavonoids extracted from Pugionium cornutum (L.) Gaerth.by macroporous resins[J].Applied chemical industry,2012,41(5):827-830. |
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| Authors: | WEN Jun-feng LIU Bu-ming |
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| Affiliation: | (School of Chemistry and Chemical Engineering,Yulin University,Yulin 719000,China) |
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| Abstract: | A method of separation and purification of flavonoids from Pugionium cornutum(L.) Gaerth.was studied.The suitable resin D-4020 was choosed by static dynamic adsorption experiments of seven macroporous resin.The adsorption experiment of D-4020 resin was done to determine the best condition.The optimal purification conditions were as follows:the sample solution concentration 0.4 mg/mL,pH 5,the sample flow rate at 2 mL/min;the elution solvent 4BV of 95 % ethanol,the flow rate at 2 mL/min.Under above condition,the purity of flavonoids could reach to 40.91%. |
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| Keywords: | Pugionium cornutum(L )Gaerth total flavonoids purification macroporous resin |
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