Humanization of lepidopteran insect-cell-produced glycoproteins |
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Authors: | Tomiya Noboru Betenbaugh Michael J Lee Yuan C |
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Affiliation: | Department of Biology, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA. ntomiya1@jhu.edu |
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Abstract: | The insect cell-baculovirus expression vector system, widely used for glycoprotein production, is not ideal for pharmaceutical glycoprotein production due to the characteristics of the N-glycans in the expressed products. Insect cells lack several enzymes required for mammalian-type N-glycan synthesis and contain a specific N-acetylglucosaminidase that stunts the growth of chains and a core alpha-1,3-fucosyltransferase that yields potentially allergenic glycoforms. Current knowledge on N-glycan processing in lepidopteran insect cells is summarized, and strategies to develop better glycoprotein expression systems suitable for pharmaceutical glycoprotein production are discussed. |
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