| 利用同源重组技术调节啤酒中SO2含量研究 |
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| 引用本文： | 赵传仕,王忠民,王德良,陆晓慧. 利用同源重组技术调节啤酒中SO2含量研究[J]. 酿酒, 2006, 33(6): 62-64 |
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| 作者姓名： | 赵传仕 王忠民 王德良 陆晓慧 |
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| 作者单位： | 1. 新疆农业大学食品学院,乌鲁木齐,830052
2. 中国食品发酵工业研究院,北京,100027 |
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| 摘 要： | 二氧化硫在啤酒中具有抗氧化的重要功能,亚硫酸盐还原酶(MET10编码)在啤酒酵母硫代谢过程中起重要作用。利用同源重组技术,采用醋酸锂转化法,将一段目的基因转入到酵母体内,从而获得一株亚硫酸盐还原酶基因突变的工业酿酒酵母。突变株通过驯养,对比发酵栓试验,结果表明在发酵结束时,突变菌株的SO2产量是出发菌株的1.5倍。
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| 关 键 词： | 同源重组 风味 啤酒酵母 |
| 文章编号： | 1002-8110（2006）06-0062-03 |
| 收稿时间： | 2005-08-03 |
| 修稿时间： | 2005-08-03 |
The Study on Regulation of Sulphur Dioxide in Beer by Homologous Recombination Techniques |
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| ZHAO Chuan-shi,WANG Zhong-min,WANG De-liang,LU Xiao-hui. The Study on Regulation of Sulphur Dioxide in Beer by Homologous Recombination Techniques[J]. Liquor Making, 2006, 33(6): 62-64 |
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| Authors: | ZHAO Chuan-shi WANG Zhong-min WANG De-liang LU Xiao-hui |
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| Abstract: | Sulphite is an important to stabilize the beer flavor because of its antioxidant activity.Sulfite reductase(encoded by MET10) plays important role in the process of sulphur metabolism.With the application of homologous recombination techniques,a resistant gene was transformed to Saccharomyces cerevisiae genome so as to inactivate MET10 by LiAc method and a new mutant strain was abtained?After domestication of mutant strain,a small scale fermentation was carried out.At the end of fermentation,the sulphite produced by the mutant strain was 1.5 fold as much as that of the original strain. |
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| Keywords: | SO2 |
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