| p53和Bcl—2／Bax在辐射诱导胸腺细胞凋亡中的作用 |
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| 引用本文： | 苏旭,万虹.p53和Bcl—2／Bax在辐射诱导胸腺细胞凋亡中的作用[J].辐射研究与辐射工艺学报,1997,15(2):119-122. |
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| 作者姓名： | 苏旭 万虹 |
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| 作者单位： | 白求恩医科大学卫生部放射生物重点实验室 |
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| 摘 要： | 采用流式细胞术对昆明小鼠接受７５ｍＧｙ和２ＧｙＸ射线全身照射后，胸腺细胞内细胞凋亡相关基因ｐ５３，Ｂｃｌ－２和Ｂａｘ蛋白的表达进行了比较研究。结果表明，７５ｍＧｙＸ射线全身照射后２４ｈ，ｐ５３基因蛋白产物的表达显著降低，而２ＧｙＸ射线全身照射后２４ｈ，ｐ５３基因蛋白表达则显著增高。
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| 关 键 词： | 细胞凋亡 电离辐射 流式细胞术 基因 胸腺细胞 |
THE EFFECTS OF p53 AND Bcl-2/Bax ON APOPTOSIS OF THYMOCYTES INDUCED BY IONIZING RADIATION |
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| Su Xu,Wan Hong,Lui Shuzheng.THE EFFECTS OF p53 AND Bcl-2/Bax ON APOPTOSIS OF THYMOCYTES INDUCED BY IONIZING RADIATION[J].Journal of Radiation Research and Radiation Processing,1997,15(2):119-122. |
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| Authors: | Su Xu Wan Hong Lui Shuzheng |
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| Abstract: | Kunming mice were whole body irradiated (WBI) with75mGy and 2Gy X rays. The expression of p53, Bcl 2 and Bax proteinswas analyzed with flow cytometry. It was found that the expression ofp53 protein showed, significantly decreased ( p< 0.005) 24 hours afterWBI with 75mGy X rays and significantly increased ( p< 0.005) after WBIwith 2 Gy X rays. The ratio of Bcl 2/Bax was significantly increased( p< 0.05) 24 hours after WBI with 75mGy X-rays and not significant changed after WBI with 2Gy. The change in expression of p53 and Bcl 2/Baxratio WBI with 75mGy and 2Gy X-rays may give partial explanation to the inhibitory effect of low dose radiation on thymocyte apoptosis. The results suggested that p53 and Bcl 2/Bax play an important role on apoptosis of thymocytes induced by ionizing radiation. |
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| Keywords: | p53 Bcl 2 Bax Apoptosis Ionizing radiation Flow cytometry(FCM) |
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