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Oxygen-Sensor-Based Simple Assay of Histamine in Fish Using Purified Amine Oxidase |
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| Authors: | MINORU OHASHI FUMIKO NOMURA MIEKO SUZUKI MEGUMI OTSUKA OSAO ADACHI NOBUHIKO ARAKAWA |
| Affiliation: | Author Ohashi is with Moritex Co., 3–1–14, Jingumae, Shibuyaku, Tokyo 150 Japan. Authors Nomura, Suzuki, Otsuka, and Arakawa are with the Dept. of Nutrition &Food Science, Ochanomizu Univ., 2–1–1, Otsuka, Bunkyo–ku, Tokyo 112 Japan. Author Adachi is with the Faculty of Agriculture, Dept. of Biological Chemistry, Yamaguchi Univ., 1677, Yoshida, Yamaguchi City, 753 Jaoan. |
| Abstract: | Oxygen consumption was measured by an oxygen sensor after addition of purified fungal amine oxidase to fish extracts. The oxidation of histamine to imidazoleacetaldehyde proceeded stoichiometrically. Based on the equimolar relationship between histamine and oxygen consumption, histamine was determined selectively by the oxygen sensor. Neither sample pretreatment removing interfering materials nor daily calibration by histamine standard was required. Histamine contents in scombroid fish were determined rapidly with good accuracy. AOAC and oxygen sensor methods showed a very high correlation (r=0.999, n=6). |
| Keywords: | fish histamine oxygen sensor amine oxidase |