谷氨酰胺转氨酶在CO2超临界流体介质与水相介质中对胶原交联作用对比

探讨了CO2超临界流体(SFC-CO2)介质中谷氨酰胺转氨酶(TGase)对胶原的交联作用,且采用差示扫描量热法(DSC)表征胶原交联效果,并与常规水相介质条件下的酶催化胶原交联反应作出对比分析。结果表明:在SCF-CO2介质中TGase催化胶原的交联反应是能发生的,酶处理后胶原的热稳定性得到了显著的提高,且与水相介质条件下酶处理的胶原相比,其热变性温度提高了10～20℃。本试验得出TGase在SCF-CO2介质中催化胶原交联反应较适宜的条件为:时间2 h、温度40℃、酶用量40～60 U/g、pH值6.0～7.0、压力8.0 MPa。

Study on the Cross-Linking of Collagen Catalyzed by Transglutaminase in CO2 -Supercritical Fluid Contrasting in Water

The effect of the collagen cross-linking which catalyzed by transglutaminase(TGase) in CO2-supercritical fluid(SCF-CO2) was investigated by Differential Scanning Calorimeter(DSC) in this paper,and which was contrasted with the cross-linking reaction in water medium.The results indicated that the cross-linking reaction of collagen catalyzed by TGase in SCF-CO2 could be occurred and the thermal stability of the treated collagen was significantly improved.In addition,comparing with the collagen catalyzed by TGase in water medium,the thermal denaturation temperature of collagen treated within SCF-CO2 medium was increased 10 to 20 ℃.The appropriate conditions of enzymatic reaction in SCF-CO2 as follows: time 2 h,temperature 40 ℃,TGase 40~60 Unit/g collagen,pH 6.0~7.0,pressure 8.0 MPa.