Transformation of bile acids and sterols by clostridia (fusiform bacteria) in Wistar rats |
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| Authors: | Kiyohisa Uchida Takashi Satoh Seiko Narushima Kikuji Itoh Haruto Takase Kazuo Kuruma Hiroyuki Nakao Nobuo Yamaga Kazuo Yamada |
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| Affiliation: | (1) Strategic Information Unit, Shionogi & Co., Ltd., Shibuya, Tokyo, Japan;(2) Department of Veterinary Medicine, Graduate School of Agriculture and Life Science, Bunkyo, Tokyo, Japan;(3) Shionogi Research Laboratories, Toyonaka, Osaka, Japan;(4) Shionogi Aburahi Laboratories, Koga, Shiga, Japan;(5) Department of Biochemistry, Faculty of Medicine, Tottori University, Yonago, Japan;(6) The Cell Research Foundation, 3-1-8, Doshomachi, Chuo-ku, 541-0045 Osaka, Japan |
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| Abstract: | The effects on bile acid and sterol transformation of clostridia (fusiform bacteria), the dominant intestinal bacteria in
rodents (ca. 1010 counts per g wet feces) were examined in Wistar rats. After inoculation of clostridia into germ-free rats and into rats previously
inoculated solely with Escherichia coli, most of the endogenous bile acids were deconjugated, and cholic acid and chenodeoxycholic acid were 7α-dehydroxylated to
deoxycholic acid and lithocholic acid, respectively. Tauro-β-muricholic acid, another major bile acid in rats, was deconjugated,
but only part of it (ca. 30%) was transformed into hyodeoxycholic acid. Cholesterol and sitosterol were also reduced to coprostanol and sitostanol,
respectively. Escherichia coli transformed neither bile acids nor sterols. These data suggest that clostridia play an imporant role in the formation of
secondary bile acids and coprostanol in rats. |
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