In vivo release of interleukin-1 beta into hypothalamic extracellular fluid in rats: effects of repeated sampling |
| |
Authors: | CP Phelps A Sahu J Oliver XL Ma PS Kalra SP Kalra RA Menzies L Chen M Denker E Horvath |
| |
Affiliation: | Department of Anatomy, University of South Florida, College of Medicine, Tampa 33612-4799, USA. |
| |
Abstract: | Interleukin-1 beta (Il-1 beta) concentrations in extracellular fluid (ECF) withdrawn at 10-min intervals through a push-pull cannula (PPC) located in the hypothalamus were studied in freely behaving male rats for 1 h at 24 and 72 h and again at 7 days after PPC implantation. Il-1 beta concentrations in ECF were similar in the latter. However, when ECF was sampled at 3 h and again 7 days after PPC implantation, Il-1 beta concentrations were greatly elevated at 7 days when compared to all other intervals. These results demonstrate how the relationships between Il-1 beta measured in ECF and the conditions of measurement appear to be integral parts of a whole intracerebral system: cytokine concentrations appear to be inextricably bound to intrahypothalamic conditions created by the sampling device presence and frequency of use. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|