| L-抗坏血酸传感器在果汁中Vc含量的测定 |
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| 引用本文： | 冯治平,黄丹,刘清斌.L-抗坏血酸传感器在果汁中Vc含量的测定[J].四川食品与发酵,2003,39(4):42-44. |
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| 作者姓名： | 冯治平 黄丹 刘清斌 |
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| 作者单位： | 四川轻化工学院生物工程系，四川轻化工学院生物工程系，四川轻化工学院生物工程系 自贡 643033，自贡 643033，自贡 643033 |
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| 摘 要： | 本文利用花椰菜组织通过交联制备L-抗坏血酸传感器,该传感器在pH6.0、0.1mol/L的KH_2PO_4-Na_2HPO_4真缓冲溶液中对Vc标准溶液的线性范围为5.0×10~(-5)×1.8×10~(-3)mol/L,响应时间1min。电极使用寿命在1个月以上。利用该传感器测定了部分水果和果汁中Vc的含量,所得结果与分光光度法(2,4-二硝基苯肼法)测得结果一致。
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| 关 键 词： | 生物传感器 酶电极 L-抗坏血酸 |
The determination of vitamin C in fruit juice by biosensor of L - ascorbic acid |
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| FENG Zhi - ping,HUANG Dan,LIU Qing - bin.The determination of vitamin C in fruit juice by biosensor of L - ascorbic acid[J].Sichuan Food and Fermentation,2003,39(4):42-44. |
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| Authors: | FENG Zhi - ping HUANG Dan LIU Qing - bin |
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| Abstract: | The cauliflower plant tissue and bovine serum albumin are cross - linked by glutaraldehyde, and coupled the plant tissue - based membrane to an oxygen electrode. The biosensor of L- ascorbic acid is prepared. It has a linear response over the range5.0×10-5 - 1.8×10-3mol/L mol/L in KH2PO4- Na2HPO4 buffer (pH = 6.0) and has over one month of lifetime.The response time is one minute. It is applied to determinate L -ascorbic acid in fruit juice and compared with that of spectrophotometric method, which is 2,4- dinitro phenyl hydrazine method. The agreement results are obtained. |
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| Keywords: | Biosensor Enzyme electrode L- ascorbic acid |
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