显微镜技术在植物细胞自噬研究中的应用

细胞自噬是真核生物对细胞内物质进行循环利用的重要途径.在植物中已发现的细胞自噬有两种形式,即微自噬和巨自噬.自噬在植物体的生长发育、免疫应答、胁迫反应、衰老等过程中具有非常重要的作用.显微镜技术是研究细胞自噬的重要手段,与生物化学和分子生物学技术相结合,荧光显微镜,激光扫描共聚焦显微镜,电子显微镜等技术的相互印证,大大地推动了对细胞自噬过程和机理的研究.本文扼要概述了显微镜技术在植物细胞自噬研究中的应用.

Application of microscopic techniques in investigating plant cell autophagy

Autophagy is the major system responsible for the degradation of organelles and cytosolic macromolecules in plant. Two types of autophagy, named microautophagy and macroautophagy, have been reported in plants. Autophagy plays an important role in plant. It is involved in plant growth, development, pathogen immune response, stress response and senescence and so on. With the development of various techniques and tools, especially the springing up of biochemical and molecular methods, the studies about cell autophagy have been greatly promoted. This review focuses on the microscopy techniques monitoring autophagy and their application in investigating autophagy.