丙泊酚对急性肺损伤大鼠 HMGB-1表达的影响

目的：探讨丙泊酚的肺保护作用及其可能作用机制。方法采用尾静脉注射内毒素建立急性肺损伤模型，丙泊酚和锌原卟啉同样经过尾静脉给药。将 Wistar 大鼠按随机数字表法分为实验对照组（C 组）、脂多糖组（L组）、脂多糖＋丙泊酚组（LP 组）。C 组尾静脉注射生理盐水；L 组尾静脉注射 LPS 7．5 mg·kg-1；LP 组尾静脉注射 LPS 7．5 mg·kg-1，同时静脉注射丙泊酚30 mg· kg-1。给药前及开始后第3、6和12 h 测定动脉血氧分压（PaO2），实验结束后观察肺湿/干重（W/D）比值、肺组织病理学检查并进行肺损伤轻重程度评分，同时观察各组大鼠肺组织高迁移率族蛋白1（HMGB-1）含量。结果实验前各组 PaO2无明显差异，注射 LPS 后 L 组 PaO2持续下降，和 C 组相比其 PaO2显著降低（P ＜0．05）。实验结束后 L 组和 C 组相比其 W/D 比值、肺组织病理学检查评分、HO-1及 HMGB-1含量显著增加（P ＜0．05）。而 LP 组和 L 组相比其 PaO2显著改善，而 W/D 比值、肺组织病理学检查评分及 HMGB-1含量显著降低（P ＜0．05）。结论丙泊酚具有肺保护作用，且该作用可能与其抑制 HMGB-1过度表达作用相关。

Effect of Propofol on Expression of HMGB-1 in Rats with Acute Lung Injury

Objective To investigate the protective effect of propofol on lung and its mecha-nism of action.Methods The endotoxin was injected into rats via the caudal vein to induce acute lung injury（ALI）.The propofol and zinc protoporphyrin were also given via tail vein injection. The Wistar rats were randomly divided into three groups：control group（group C）,lipopolysac-charide（LPS）group（group L）,and LPS＋propofol group（group LP）.Saline solution was injected into rats of control group via caudal vein;lipopolysaccharide （7.5 mg· kg-1 ）was injected into rats of LPS group via caudal vein;lipopolysaccharide （7.5 mg· kg-1 ）and propofol （30 mg· kg-1 ）were injected into rats of LPS＋propofol group via caudal vein at the same time.The PaO2 was measured before and at 3,6 and 12 hours after treatment.Lung wet/dry weight ratio,lung in-jury score and high mobility group box-1（HMGB-1）expression were measured after the experi-ment.Results There were no significant differences in the PaO2 among the three groups before the experiment.After LPS injection,PaO2 in group L continuously decreased and was significantly lower than that in group C（P 〈0.05）.After the experiment,lung wet/dry weight ratio,lung inju-ry score and HMGB-1 content in group L were significantly increased as compared with group C （P 〈0.05）.Compared with group L,the PaO2 was obviously improved and lung wet/dry weight ratio,lung injury score and HMGB-1 content were significantly decreased in group LP（P 〈0.05）. Conclusion Propofol can protect lung from endotoxin-induced acute lung injury through inhibi-ting the excessive expression of HMGB-1.