| 抑制烟草花叶病毒活性植物的筛选 |
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| 引用本文： | 林中正,谢娇枚,李基光,周鹏,魏润洁,肖启明.抑制烟草花叶病毒活性植物的筛选[J].中国烟草,2013(6):89-92. |
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| 作者姓名： | 林中正 谢娇枚 李基光 周鹏 魏润洁 肖启明 |
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| 作者单位： | [1]湖南农业大学生物安全科学技术学院,长沙410128 [2]江苏省淮安市农业委员会,江苏淮安223001 [3]国家植物功能成分利用工程技术研究中心,长沙410128 [4]湖南省西瓜甜瓜研究所,长沙410125 |
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| 基金项目： | 湖南省烟草专卖局项目（09-11Aa29） |
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| 摘 要： | 本研究采用半叶枯斑法和烟草病害分级标准，对抗TMV的活性植物进行筛选，系统测定了艾蒿、板蓝根、柴胡等24种植物的粗提取物对烟草花叶病毒（TMV）的抑制效果，包括体外钝化TMV作用、抑制TMV初侵染；并采用盆栽法，研究植物粗提取物对烟草花叶病的保护和治疗作用等。试验表明，银杏、栀子、商陆、赤芍等植物粗提取物的综合防治效果较好，有进一步开发和利用的前景。
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| 关 键 词： | TMV 抗病毒活性 植物提取物 烟草 |
The Screening of Plants Inhibiting Tobacco Mosaic Virus Activity |
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| LIN Zhongzheng,XIE Jiaomei,LI Jiguang,ZHOU Peng,WEI Runjie,XIAO Qiming.The Screening of Plants Inhibiting Tobacco Mosaic Virus Activity[J].China Tobacco,2013(6):89-92. |
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| Authors: | LIN Zhongzheng XIE Jiaomei LI Jiguang ZHOU Peng WEI Runjie XIAO Qiming |
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| Affiliation: | 1. College of Bio-safety Science and Technology, Hunan Agricultural University, Changsha 410128, China; 2. Huai＇an Agriculture Commission, Huai＇an, Jiangsu 223001, China; 3.National Research Center of Engineering Technology for Utilization of Botanical Functional ingredients, Changsha 410128, China; 4. Hunan Institute of Watermelon and Muskmelon, Changsha 410125, China) |
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| Abstract: | In this paper, screening of plants inhibiting Tobacco Mosaic Virus （TMV） activity was achieved under the disease measuring standard with half leaf lesion experiment. Botanical extracts were obtained from 24 kinds of plants, including Artemisia Argyi, Radix Isatis, Bupleurum, etc, and their inhibiting effect on TMV activity were tested by passive activation of TMV in vitro and inhibiting TMV primary infection. Pot experiments were used to investigate the protection and therapeutic effect of virus. The results indicated that the abstracts from ginkgo, gardenia, phytolacca and red peony root had great inhibiting effect on TMV, which is promising for further development and utilization. |
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| Keywords: | tobacco mosaic virus antiviral activity plant extract tobacco |
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