| 黑曲霉发酵液中脂肪酸化学成分的研究 |
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| 引用本文： | 王嫱,冯焕鹏,刘吴娟,李祝,陈青.黑曲霉发酵液中脂肪酸化学成分的研究[J].食品工程,2012(2):31-33. |
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| 作者姓名： | 王嫱 冯焕鹏 刘吴娟 李祝 陈青 |
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| 作者单位： | 1. 贵州大学生命科学学院,贵阳,550025
2. 贵州大学化学与化工学院,贵阳,550025 |
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| 基金项目： | 毕节地区马铃薯产业科技合作专项项目，贵州省农业攻关计划项目，贵州省科学技术基金项目 |
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| 摘 要： | 目的：分析黑曲霉（Aspergillusnigerxj）发酵液中的脂肪酸成分。方法：液体发酵培养黑曲霉0spergillusnigerxj），发酵液通过石油醚萃取，硅胶柱层析，石油醚-乙酸乙酯洗脱，得到的脂肪酸经甲酯化处理，通过气相色谱一质谱联用技术对其进行鉴定。结果：鉴定出4种脂肪酸成分。结论：黑曲霉（Aspergillusniger巧）发酵液中的脂肪酸成分主要为亚油酸、6-十八碳烯酸、14-甲基十五烷酸和硬脂酸。
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| 关 键 词： | 黑曲霉 发酵液 挥发油 气相色谱-质谱法 |
Analysis of the fatty acid from Aspergillus niger xj fermentation broth |
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| WANG Qiang,FENG Huan-peng,LIU Wu-juan,LI Zhu,CHEN Qing.Analysis of the fatty acid from Aspergillus niger xj fermentation broth[J].Food Engineering,2012(2):31-33. |
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| Authors: | WANG Qiang FENG Huan-peng LIU Wu-juan LI Zhu CHEN Qing |
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| Affiliation: | 1(College of life science,Guizhou university,Guiyang 550025,China) 2(The chemistry department of science college of Guizhou university,Guiyang 550025,China) |
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| Abstract: | Objective:To study chemical constituents of fatty acid from A Aspergillus niger xj fermentation broth.Methods:After being cultured in PDA liquid medium,the fatty acid from Aspergillus niger xj was extracted by petroleum ether from the fermentation broth,separated by silica gel column chromatography method,eluted with petroleum ether and ethyl acetate,then separated and analysed by GC-MS.Results:4 compounds were separated and identified.Conclusion The major fatty acids in Aspergillus niger xj fermentation broth including octadecadienoic acid,octadecenoic acid,pentadecanoic acid and octadecanoic acid. |
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| Keywords: | Aspergillus nigerxj fermentation broth fatty acid GC-MS |
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