| 嗜热真菌发酵产木聚糖酶培养基的优化及部分酶学性质的研究 |
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| 引用本文： | 张新峰,王伟平,汪蓉,王琛琛,阳飞,张华山.嗜热真菌发酵产木聚糖酶培养基的优化及部分酶学性质的研究[J].食品工业科技,2011(10):249-252. |
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| 作者姓名： | 张新峰 王伟平 汪蓉 王琛琛 阳飞 张华山 |
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| 作者单位： | 1. 发酵工程省部共建教育部重点实验室 湖北工业大学,湖北武汉,430068
2. 湖北大学知行学院,湖北武汉,430011 |
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| 摘 要： | 采用响应面法对嗜热真菌发酵产木聚糖酶的培养基进行优化以及对该酶液的基本性质进行研究。首先对初始培养基中的8种影响因素进行Plackett—Burman设计，筛选出3种重要影响因素，即玉米芯、尿素和KH2PO4；再利用最陡爬坡实验为中心组合实验确定最大响应区间，最后经响应面分析获得最优化结果：玉米芯浓度为4．570％（w／v），尿素浓度为1．797％（w/v），KH2PO4浓度为0．497％（w／v），酶活提高了18％。该酶对玉米芯为底物的专一性较好，最适酶促反应温度为65℃且在该温度下较稳定，最适反应pH为6．8，并且pH稳定性较好。
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| 关 键 词： | 嗜热真菌 木聚糖酶 优化 酶学性质 |
Optimization of fermentation medium for thermophilic fungal xylanase and study on some enzymatic properties |
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| ZHANG Xin-feng,WANG Wei-ping,WANG Rong,WANG Chen-chen,YANG Fei,ZHANG Hua-shan.Optimization of fermentation medium for thermophilic fungal xylanase and study on some enzymatic properties[J].Science and Technology of Food Industry,2011(10):249-252. |
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| Authors: | ZHANG Xin-feng WANG Wei-ping WANG Rong WANG Chen-chen YANG Fei ZHANG Hua-shan |
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| Affiliation: | ZHANG Xin-feng1,WANG Wei-ping1,WANG Rong2,WANG Chen-chen1,YANG Fei1,ZHANG Hua-shan1,(1. Key Laboratory of Fermentation Technology,Ministry of Education,Hubei University of Technology,Wuhan 430068,China,2. Zhixing College of Hubei University,Wuhan 430011,China) |
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| Abstract: | Fermentation medium of thermophilic fungal for xylanase production was optimized by response surface method and the basic properties of the enzyme solution were studied. First,eight kinds of factors of the initial medium was adopted by the Plackett-Burman design,and selected three kinds of important factors,namely,corn cob,urea and KH2PO4,and then the path of steepest ascent was adopted for the central composite experiment to determine the maximum response interval. Finally response surface optimization res... |
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| Keywords: | Thermophilic fungi xylanase optimization enzymatic properties |
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