| 红酵母番茄红素提取工艺优化 |
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| 引用本文： | 王海兵,吴晓英,刘世龙,徐莹莹.红酵母番茄红素提取工艺优化[J].食品科学,2011,32(16):45-48. |
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| 作者姓名： | 王海兵 吴晓英 刘世龙 徐莹莹 |
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| 作者单位： | 华南理工大学生物科学与工程学院 |
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| 基金项目： | 华南理工大学国家大学生创新性实验计划项目(091056154) |
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| 摘 要： | 从破壁方法、浸提溶剂及提取条件等方面对黏红酵母番茄红素提取工艺进行优化研究。采用单因素试验对破壁方法及浸提溶剂进行选择，结果表明热酸法是黏红酵母破壁提取番茄红素的最佳方法，丙酮-乙酸乙酯(1:1)混合液是理想的提取溶剂。采用正交试验方法对液料比、提取温度和提取时间等番茄红素提取条件进行优化，得到适宜的提取条件为丙酮-乙酸乙酯(1:1)溶剂添加量60mL/g、提取温度30℃、提取时间3h。在此提取工艺下，得到红酵母番茄红素提取量为4.55mg/g，比未优化时的3.22mg/g增加了41.30%。
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| 关 键 词： | 红酵母 番茄红素 提取工艺 |
Optimization of Extraction Process for Lycopene from Rhodotorula glutins |
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| WANG Hai-bing,WU Xiao-ying,LIU Shi-long,XU Ying-ying.Optimization of Extraction Process for Lycopene from Rhodotorula glutins[J].Food Science,2011,32(16):45-48. |
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| Authors: | WANG Hai-bing WU Xiao-ying LIU Shi-long XU Ying-ying |
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| Affiliation: | (School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China) |
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| Abstract: | Cell wall disruption followed by organic solvent extraction was used to extract lycopene from Rhodotorula glutins. The optimal cell wall disruption method and extraction solvent were found to be hot acid treatment and acetone-ethyl acetate (1:1), respectively. Three extraction conditions such as material-to-liquid ratio, temperature and time were optimized by orthogonal array design to be 1:60 (g/mL), 30 ℃ and 3 h, respectively. Under these conditions, the extraction rate of lycopene was 4.55 mg/g, 41.30% higher than before optimization. |
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| Keywords: | Rhodotorula lycopene extraction process |
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