| 灵芝多糖树脂法脱色工艺优化 |
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| 引用本文： | 罗玺,唐庆九,张劲松,周帅,吴迪,刘艳芳,王琪,乔彦茹,杨焱,赵明文.灵芝多糖树脂法脱色工艺优化[J].食品科学,2011,32(16):5-10. |
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| 作者姓名： | 罗玺 唐庆九 张劲松 周帅 吴迪 刘艳芳 王琪 乔彦茹 杨焱 赵明文 |
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| 作者单位： | 1.国家食用菌工程技术研究中心，农业部应用真菌资源与利用重点开放实验室，上海市农业遗传重点开放实验室，上海市农业科学院食用菌研究所
2.南京农业大学生命科学学院微生物系
3. 河北省食用菌研究所 |
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| 基金项目： | 国家“863”计划项目(2007AA10Z341) |
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| 摘 要： | 利用大孔树脂对灵芝多糖进行脱色。比较10种大孔树脂在脱色方面的性能，以灵芝多糖的脱色率和多糖保留率为考察指标，结果发现D303树脂的脱色效果最佳，通过单因素试验和正交试验对D303树脂的灵芝多糖脱色各种工艺参数进行优化，得到D303树脂静态脱色的最佳参数为在样品上样质量浓度15mg/mL、pH6、脱色温度50℃、脱色时间7h条件下，灵芝多糖溶液的脱色率可达91.89%，多糖保留率75.28%；D303树脂动态脱色的最佳参数为上样流速3BV/h、每毫升树脂上样量150mg，此条件下灵芝多糖溶液的脱色率可达92.01%，多糖保留率71.85%。研究表明D303树脂适合应用于灵芝多糖的脱色工艺。
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| 关 键 词： | 灵芝 多糖 脱色 树脂 |
Optimization of Decolorization Technology of Ganoderma lucidum Polysaccharides by Macroporous Resin Adsorption Method |
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| LUO Xi,TANG Qing-jiu,ZHANG Jin-song,ZHOU Shuai,WU Di,LIU Yan-fang,WANG Qi,QIAO Yan-ru,YANG Yan,ZHAO Ming-wen.Optimization of Decolorization Technology of Ganoderma lucidum Polysaccharides by Macroporous Resin Adsorption Method[J].Food Science,2011,32(16):5-10. |
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| Authors: | LUO Xi TANG Qing-jiu ZHANG Jin-song ZHOU Shuai WU Di LIU Yan-fang WANG Qi QIAO Yan-ru YANG Yan ZHAO Ming-wen |
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| Affiliation: | 1. National Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization, Ministry
of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural
Sciences, Shanghai 201106, China；2. Department of Microbiology, College of Life Sciences, Nanjing Agricultural University,
Nanjing 210095, China；3. Institute of Edible Fungi of Hebei Province, Shijiazhuang 050035, China |
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| Abstract: | The purpose of this study was to develop a macroporous resin adsorption method for the decolorization of Ganoderma lucidum polysaccharides.Ganoderma lucidum polysaccharides with a molecular weight between 10 kD and 500 kD were obtained by extracting Ganoderma lucidum fruiting bodies twice with boiling water,concentrating and ultrafiltrating with different MWCO(10 kD and 500 kD) membranes.Ten types of macroporous resin were compared for their effectiveness in decolorizing the polysaccharides.Based on decolor... |
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| Keywords: | Ganoderma lucidum polysaccharide decoloration resin |
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