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| 环介导恒温核酸扩增法的研究和技术方法 | |
| 引用本文： | 李彦媚, 赵喜红, 徐泽智, 徐振波. 环介导恒温核酸扩增法的研究和技术方法[J]. 食品工业科技, 2011, (11): 514-518. DOI: 10.13386/j.issn1002-0306.2011.11.036 |
| 作者姓名： | 李彦媚 赵喜红 徐泽智 徐振波 |
| 作者单位： | 1. 广州医学院,广东广州,510120 2. 武汉工程大学化工与制药学院绿色化工过程省部共建教育部重点实验,湖北武汉,430073 3. 南海水产研究所,广东广州,510300 4. 华南理工大学轻工与食品学院,广东广州510640／美国马里兰大学生物医学科学系,马里兰21201 |
| 摘 要： | 核酸扩增是生命科学领域最具价值的工具之一,在临床微生物检测、传染性疾病以及基因诊断方面具有独特的应用价值。传统的检测方法均存在一定的局限性,因此基于核酸扩增的检测方法具有广阔的应用前景。环介导恒温核酸扩增法（loop-mediatedisothermalamplificationofDNA,简称LAMP）是一种新型的核酸扩增方法,由于其特异性和灵敏度高、简便快捷且成本低廉,因此在可预见的将来,LAMP技术将在核酸扩增领域逐渐取代PCR反应,推动检测技术向更快捷简便,更精确廉价的方向发展。 |
| 关 键 词： | 环介导恒温核酸扩增法 核酸扩增 临床检测 |
Development on the techniques of loop-mediated isothermal amplification |
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| LI Yan-mei,ZHAO Xi-hong,XU Ze-zhi,XU Zhen-bo,,(.Guangzhou Medical College,Guangzhou ,China,.Key Laboratory for Green Chemical Process of Ministry of Education,School of Chemical Engineering , Pharmacy,Wuhan Institute of Technology,Wuhan ,.South China Fishery Institute,Guangzhou ,.College of Light Industry , Food Sciences,South China University of Technology,Guangzhou . Development on the techniques of loop-mediated isothermal amplification[J]. Science and Technology of Food Industry, 2011, 0(11): 514-518. DOI: 10.13386/j.issn1002-0306.2011.11.036 | |
| Authors: | LI Yan-mei ZHAO Xi-hong XU Ze-zhi XU Zhen-bo (.Guangzhou Medical College Guangzhou China .Key Laboratory for Green Chemical Process of Ministry of Education School of Chemical Engineering Pharmacy Wuhan Institute of Technology Wuhan .South China Fishery Institute Guangzhou .College of Light Industry Food Sciences South China University of Technology Guangzhou |
| Affiliation: | LI Yan-mei1,ZHAO Xi-hong2,XU Ze-zhi3,XU Zhen-bo4,5,(1.Guangzhou Medical College,Guangzhou 510120,China,2.Key Laboratory for Green Chemical Process of Ministry of Education,School of Chemical Engineering and Pharmacy,Wuhan Institute of Technology,Wuhan 430073,3.South China Fishery Institute,Guangzhou 510300,4.College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,5.Department of Microbial Pathogenesis,Dental School,University of Maryland,Baltimore,... |
| Abstract: | Clinical detection based on genetic amplification is one of the most valuable tools in bioscience,which had been proved to be applicable in detection of clinical microbes,infectious diseases and gene diagnosis.Limitation on traditional detection methods posed significant obstacles for their broad application.A novel nucleic acid amplification method,designated loop-mediated isothermal amplification(LAMP),known as a rapid,low-cost,easy operating,highly sensitive and specific detection method,had been reporte... |
| Keywords: | loop-mediated isothermal amplification nucleic acid amplification clinical detection |
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