双水相萃取法分离纯化狗枣猕猴桃叶总黄酮

采用聚乙二醇-硫酸钠双水相体系分离纯化狗枣猕猴桃叶黄酮,确定其双水相体系组成24%PEG400-15%Na2SO4,并通过单因素试验和正交试验探讨黄酮粗提液质量浓度、KCl添加量、pH值和温度对萃取效果的影响。结果表明:黄酮粗提液质量浓度和pH值对黄酮萃取效果影响最大,其次为KCl添加量,温度的影响较小;最佳萃取条件为粗提液质量浓度C0、KCl添加量2%、pH9、25℃。在此条件下,狗枣猕猴桃叶黄酮主要分布在上相,分配系数为24.31,萃取率达到96.90%。此方法操作简单方便、成本低,是黄酮类化合物分离纯化的一种有效方法。

Optimization of Aqueous Two-phase Extraction of Total Flavonoids from Actinidia kolomikta Leaves

An aqueous two-phase system composed of 24% PEG400 and 15% Na2SO4 was used to extract total flavonoids from the crude ethanol extract of Actinidia kolomikta leaves.The effects of crude extract concentration,KCl amount,pH and extraction temperature on the extraction efficiency of total flavonoids were explored by one-factor-at-a-time and orthogonal array design methods.The results showed that crude extract concentration and pH were major affecting factors and KCl,and extraction temperature exhibited less eff...