| 苯酚-硫酸法测定海湾扇贝多糖方法的研究 |
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| 引用本文： | 佟海菊,张志胜,孙克岩,刘亚南. 苯酚-硫酸法测定海湾扇贝多糖方法的研究[J]. 食品工业科技, 2011, 0(10): 447-448,452 |
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| 作者姓名： | 佟海菊 张志胜 孙克岩 刘亚南 |
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| 作者单位： | 河北农业大学食品科技学院,河北保定,071001 |
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| 基金项目： | 国家海洋公益性行业科研专项(200805046) |
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| 摘 要： | 以葡萄糖为标准品，优化苯酚-硫酸法测定海湾扇贝多糖含量的条件，并对该法的精密度、加标回收率等进行了研究，得出最佳显色条件为：苯酚用量1．0mL，浓硫酸用量5．0mL，室温放置30min，在480nm处测定吸光度。结果表明．此方法是测定海湾扇贝多糖含量的一种稳定性好、回收率高的简便方法。
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| 关 键 词： | 苯酚-硫酸法 海湾扇贝多糖 含量测定 |
Study on determination of polysaccharides content in argopectens irradias by phenol-sulfuric acid method |
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| TONG Hai-ju,ZHANG Zhi-sheng,SUN Ke-yan,LIU Ya-nan. Study on determination of polysaccharides content in argopectens irradias by phenol-sulfuric acid method[J]. Science and Technology of Food Industry, 2011, 0(10): 447-448,452 |
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| Authors: | TONG Hai-ju ZHANG Zhi-sheng SUN Ke-yan LIU Ya-nan |
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| Affiliation: | TONG Hai-ju,ZHANG Zhi-sheng,SUN Ke-yan,LIU Ya-nan(College of Food Science and Technology,Agricultural University of Hebei,Baoding 071001,China) |
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| Abstract: | To optimize the condition of determining polysaccharides content by phenol-sulfuric acid method,using glucose as the standard sample. The precision and the recovery rate were studied,the better condition was as follows:1.0mL 6% solution of phenol,sulfuric acid 5.0mL,room-temperature for 30min,the absorbency rate was tested in wavelength 480nm. The result showed that the method can be used to determine the argopectens irradias polysaccharides simply and accurately. |
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| Keywords: | phenol-sulfuric acid method argopectens irradias polysaccharides content determination |
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