| 动物肌肉组织基因组DNA两种提取方法的比较 |
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| 引用本文： | 徐伟丽, 杜明, 李启明, 汪家琦, 张伟洋, 马莺. 动物肌肉组织基因组DNA两种提取方法的比较[J]. 食品工业科技, 2011, (12): 81-84. DOI: 10.13386/j.issn1002-0306.2011.12.002 |
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| 作者姓名： | 徐伟丽 杜明 李启明 汪家琦 张伟洋 马莺 |
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| 作者单位： | 1. 哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨,150090
2. 新希望乳业控股有限公司技术中心,四川成都,610023 |
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| 基金项目： | 四川省应用基础研究(2009JY0101) |
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| 摘 要： | 以猪、牛、羊、鸡等动物新鲜冷冻样品为实验材料,采用SDS法和异硫氰酸胍法提取动物肌肉组织基因组DNA,对提取的DNA进行光密度、琼脂糖凝胶电泳和PCR检测,比较了这两种方法的提取效果。结果表明:采用SDS法和异硫氰酸胍法均能从动物肌肉组织提取到完整的基因组DNA,均可满足PCR等后继分子生物学实验的要求。但是异硫氰酸胍法提取的基因组在纯度和浓度及稳定性方面优于SDS法,且操作简单,耗时短,更利于快速检测。
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| 关 键 词： | 动物肌肉组织 基因组DNA 提取 聚合酶链式反应 |
Comparison of the effects of two methods for four animal musclar tissue DNA extraction |
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| XU Wei-li,DU Ming,LI Qi-ming,WANG Jia-qi,ZHANG Wei-yang,MA Ying. Comparison of the effects of two methods for four animal musclar tissue DNA extraction[J]. Science and Technology of Food Industry, 2011, 0(12): 81-84. DOI: 10.13386/j.issn1002-0306.2011.12.002 |
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| Authors: | XU Wei-li DU Ming LI Qi-ming WANG Jia-qi ZHANG Wei-yang MA Ying |
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| Affiliation: | XU Wei-li1,DU Ming1,LI Qi-ming2,WANG Jia-qi2,ZHANG Wei-yang1,MA Ying1,(1. School of Food Science and Engineering,Harbin Institute of Technology,Harbin 150090,China,2. Technique Center,New Hope Dairy Holdings Co.,Ltd.,Chengdu 610023,China) |
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| Abstract: | Genomic DNA of musclar tissues from swine,cattle,goat and chicken were extracted through SDS method and GuSCN method,respectively. The DNA was detected by optical density,agarosegel electrophoresis and polymerase chain reaction. The extraction effects of two methods were compared. Results indicated that high quality genomic DNA was separately extracted from muscle tissue of 4 animals by the SDS method and the GuSCN method,and meeted the requirements of varieties of molecular biology experiments. But the GuS... |
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| Keywords: | animal musclar tissues genomic DNA extraction PCR |
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