| 紫外线和He—Ne激光诱变产植酸酶黑曲霉菌株的研究 |
| |
| 引用本文： | 张卫兵,郭爱莲,甘伯中.紫外线和He—Ne激光诱变产植酸酶黑曲霉菌株的研究[J].食品工业科技,2011(10):228-230. |
| |
| 作者姓名： | 张卫兵 郭爱莲 甘伯中 |
| |
| 作者单位： | 甘肃农业大学食品科学与工程学院 甘肃农业大学研究测试中心 甘肃省干酪素工程技术研究中心,甘肃兰州,730070 |
| |
| 基金项目： | 甘肃省农业生物技术专项项目(GNSW-2006-14); 甘肃省科技重大专项项目(0702NKDA034) |
| |
| 摘 要： | 采用He—Ne激光（波长632nm，功率10mW）和紫外线对植酸酶产生茵黑曲霉Px的孢子和原生质体进行诱变。结果表明：原生质体对紫外线诱变的耐受能力比孢子强，而对He—Ne激光诱变的耐受能力比孢子弱。经诱变后筛选出一株突变菌株L2—2，植酸酶产量为9274IU／mL，是出发菌株的1．51倍，传代实验表明该菌株植酸酶产量稳定。
|
| 关 键 词： | 植酸酶 He—Ne激光 原生质体 孢子 |
Mutation breeding of Aspergillus Niger with high phytase activity by ultra violet and He-Ne laser irradiation |
| |
| ZHANG Wei-bing,GUO Ai-lian,GAN Bo-zhong.Mutation breeding of Aspergillus Niger with high phytase activity by ultra violet and He-Ne laser irradiation[J].Science and Technology of Food Industry,2011(10):228-230. |
| |
| Authors: | ZHANG Wei-bing GUO Ai-lian GAN Bo-zhong |
| |
| Affiliation: | ZHANG Wei-bing,GUO Ai-lian,GAN Bo-zhong(College of Food Science and Engineering,Gansu Agricultural University,Analysis and Research Center of Gansu Agricultural University,Gansu Casein Engineering Technical Research Center Lanzhou,Lanzhou 730070,China) |
| |
| Abstract: | The protoplasts and spores of the origin strain Px were treated by laser and UV radiation to obtain strain with high phytase activity. Mutant L2-2 was obtained,whose phytase activity was 9274 IU/mL,as 1.51 times as the original strain. A good application and exploitation perspective of this strain was shown. |
| |
| Keywords: | phytase He-Ne laser protoplasts spore |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
