| 紫外线和He-Ne激光诱变产植酸酶黑曲霉菌株的研究 |
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| 引用本文： | 张卫兵, 郭爱莲, 甘伯中. 紫外线和He-Ne激光诱变产植酸酶黑曲霉菌株的研究[J]. 食品工业科技, 2011, (10): 228-230. DOI: 10.13386/j.issn1002-0306.2011.10.036 |
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| 作者姓名： | 张卫兵 郭爱莲 甘伯中 |
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| 作者单位： | 甘肃农业大学食品科学与工程学院 甘肃农业大学研究测试中心 甘肃省干酪素工程技术研究中心,甘肃兰州,730070 |
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| 基金项目： | 甘肃省农业生物技术专项项目(GNSW-2006-14); 甘肃省科技重大专项项目(0702NKDA034) |
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| 摘 要： | 采用He-Ne激光（波长632nm,功率10mW）和紫外线对植酸酶产生菌黑曲霉Px的孢子和原生质体进行诱变。结果表明:原生质体对紫外线诱变的耐受能力比孢子强,而对He-Ne激光诱变的耐受能力比孢子弱。经诱变后筛选出一株突变菌株L2-2,植酸酶产量为9274IU/mL,是出发菌株的1.51倍,传代实验表明该菌株植酸酶产量稳定。
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| 关 键 词： | 植酸酶 He-Ne激光 原生质体 孢子 |
Mutation breeding of Aspergillus Niger with high phytase activity by ultra violet and He-Ne laser irradiation |
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| ZHANG Wei-bing,GUO Ai-lian,GAN Bo-zhong. Mutation breeding of Aspergillus Niger with high phytase activity by ultra violet and He-Ne laser irradiation[J]. Science and Technology of Food Industry, 2011, 0(10): 228-230. DOI: 10.13386/j.issn1002-0306.2011.10.036 |
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| Authors: | ZHANG Wei-bing GUO Ai-lian GAN Bo-zhong |
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| Affiliation: | ZHANG Wei-bing,GUO Ai-lian,GAN Bo-zhong(College of Food Science and Engineering,Gansu Agricultural University,Analysis and Research Center of Gansu Agricultural University,Gansu Casein Engineering Technical Research Center Lanzhou,Lanzhou 730070,China) |
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| Abstract: | The protoplasts and spores of the origin strain Px were treated by laser and UV radiation to obtain strain with high phytase activity. Mutant L2-2 was obtained,whose phytase activity was 9274 IU/mL,as 1.51 times as the original strain. A good application and exploitation perspective of this strain was shown. |
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| Keywords: | phytase He-Ne laser protoplasts spore |
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