| 产2-酮基-D-葡萄糖酸菌Serratiasp．BK-98的分离及其培养条件优化 |
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| 引用本文： | 张炜,谢志鹏,张建国. 产2-酮基-D-葡萄糖酸菌Serratiasp．BK-98的分离及其培养条件优化[J]. 食品工业科技, 2011, 0(10): 264-267,372 |
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| 作者姓名： | 张炜 谢志鹏 张建国 |
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| 作者单位： | 浙江大学生物化学研究所,浙江杭州,310058 |
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| 摘 要： | 从土壤中分离得到一株2-酮基-D-葡萄糖酸（2-KDG）产生菌，综合16SrDNA序列和系统进化分析确定该菌属于沙雷氏菌属（Serratia），命名为Serratiasp．BK-98。采用PIackett—Burman（PB）实验设计，从影响2-酮基-D-葡萄糖酸生物合成条件的14个因素中筛选出具有显著效应的3个因子：装液量、发酵时间和初始pH。在此基础上通过中心组合设计实验（central composite design，CCD）和响应面分析（response surface methodology，RSM）确定了装液量、发酵时间和初始DH的最适值分别为6．6mL、57．9h和5．0。在优化条件下．2-KDG的100mL摇瓶发酵产量达到了187．8g／L，100L发酵罐产量达到了192．2g／L．分别较优化前提高了142．6％和148．3％．这两个实验结果均与模型的预测值191．4g／L非常接近。
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| 关 键 词： | 培养条件 2-酮基-D-葡萄糖酸 优化 响应面法 Serratia sp．BK-98 |
Optimization of culture conditions for producing 2-keto-D-gluconic acid by an isolated strain of Serratia sp. BK-98 |
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| ZHANG Wei,XIE Zhi-peng,ZHANG Jian-guo. Optimization of culture conditions for producing 2-keto-D-gluconic acid by an isolated strain of Serratia sp. BK-98[J]. Science and Technology of Food Industry, 2011, 0(10): 264-267,372 |
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| Authors: | ZHANG Wei XIE Zhi-peng ZHANG Jian-guo |
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| Affiliation: | ZHANG Wei,XIE Zhi-peng,ZHANG Jian-guo(Institute of Biochemistry,Zhejiang University,Hangzhou 310058,China) |
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| Abstract: | A strain capable of producing 2-keto-D-gluconic acid(2-KDG) was isolated from soil. The phylogenetic analysis based on 16S rDNA suggested the isolate was assigned to genus Serratia and named Serratia sp. BK-98. Medium loading volume,fermentation time and initial pH were found to be most significant factors affecting 2-KDG production using Plackett-Burman(PB) design and their values were optimized to be 6.6mL in a 100mL Erlenmeyer flask of medium loading volume,57.9h of fermentation time and 5.0 of initial p... |
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| Keywords: | culture conditions 2-keto-D-gluconic acid optimization response surface methodology Serratia sp. BK-98 |
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