Ethical implications for early pregnancy of the fetus as patient: a basic ethical concept in fetal medicine |
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Authors: | FA Chervenak LB McCullough |
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Affiliation: | Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy. |
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Abstract: | A new and rapid method is proposed for extraction of non-polar lipids from tissues where they are present as abundant components which can interfere with the usual procedures of lipid extraction and TLC separation, and hamper, in particular, sulphatide visualization. A solvent more hydrophobic than chloroform, i.e. n-hexane, was utilized to remove the neutral lipids from samples of female rabbit parotid gland, and the n-hexane phase was used for TLC which showed considerable amounts of cholesterol esters, in addition to triglycerides, diglycerides and monoglycerides. The methanol phase, now devoid of non-polar lipids, was utilized to prepare TLC plates in order to separate and visualize the polar lipid fractions, in particular the sulphatides. |
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