四大产区莼菜黄酮类物质组成分析及抗氧化、抑菌活性研究

为研究莼菜黄酮类物质组成及抗氧化、抑菌体外功能活性,利用紫外-可见分光光度法和高效液相色谱法分别测定中国四大产区莼菜总黄酮、黄酮类物质组成及含量,2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS),1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH),铁还原/抗氧化能力(ferric reducing/antioxidant power,FRAP)三种抗氧化法评价各产区莼菜黄酮抗氧化能力,滤纸片扩散法测定莼菜黄酮对变形杆菌(Proteus vulgaris)、大肠杆菌(Escherichia coli)和铜绿假单胞菌(Pseudomonas aeruginosa)的抑菌效果。结果显示:石柱莼菜总黄酮含量最高,为(42. 505±0. 458) mg/g,西湖莼菜最低,为(15. 845±0. 119) mg/g;供试材料中共检测到7种黄酮类物质,主要为异鼠李素、槲皮素和山奈酚3种;各产区莼菜均具有较好的抗氧化活性及不同程度的抑菌活性,且其大小变化顺序一致,均为:石柱莼菜﹥利川莼菜﹥雷波莼菜﹥西湖莼菜。该结果为莼菜黄酮类物质的进一步研究提供参考。

Composition analysis and antioxidant and antibacterial activities of flavonoids of Brasenia schreberi in four main areas

In order to study the composition of water shield flavonoids and their antioxidant and antibacterial activities in vitro,in this study,the composition and content of flavonoid in water shield from four major producing areas in China were determined by ultraviolet-visible spectrophotometry(UV-VIS)and high performance liquid chromatography(HPLC).Three antioxidant methods,DPPH,ABTS and FRAP were used to evaluate the antioxidant capacity of flavonoids in water shield from various regions.The filter paper diffusion method was used to determine the antibacterial effects of flavonoids from water shield on Proteus vulgaris,Escherichia coli and Pseudomonas aeruginosa.The results showed that the content of total flavonoids in Shizhu water shield was the highest(42.505±0.458)mg/g,and West Lake was the lowest(15.845±0.119)mg/g.A total of 7 flavonoids were detected in the test materials,mainly isorhamnetin,quercetin and kaempferol.Water shield in each production area has good antioxidant activity and different degrees of antibacterial activity,and the order of their size changes is the same:all are:Shizhu﹥Lichuan﹥Leibo﹥West Lake.This will provide reference data for further research on water shield flavonoids.