| 离子液体双水相中铕离子与牛血清白蛋白作用的研究 |
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| 引用本文： | 陈琳琳,汤秀琴,邓凡政.离子液体双水相中铕离子与牛血清白蛋白作用的研究[J].化工科技,2011,19(3):13-15,30. |
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| 作者姓名： | 陈琳琳 汤秀琴 邓凡政 |
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| 作者单位： | 淮北师范大学化学与材料科学学院,安徽淮北,235000 |
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| 基金项目： | 安徽省教育厅自然科学研究重点项目(KJ2008A123) |
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| 摘 要： | 运用荧光光谱,研究了在离子液体双水相中铕离子(Eu3+)与牛血清白蛋白(BSA)的相互作用.研究发现铕离子对牛血清白蛋白有较强的荧光猝灭作用,且为静态猝灭类型.根据双对数方程处理荧光猝灭数据得到了铕离子与牛血清白蛋白在不同温度下的结合常数Ka和结合位点数n.通过热力学方程求得了铕离子与牛血清白蛋白作用的热力学参数,表明...
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| 关 键 词： | 铕离子 牛血清白蛋白 荧光猝灭 相互作用 离子液体双水相 |
Study on interaction between Eu3+and BSA in ionic liquid aqueous two-phase |
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| CHEN Lin-lin,TANG Xiu-qin,DENG Fan-zheng.Study on interaction between Eu3+and BSA in ionic liquid aqueous two-phase[J].Science & Technology In Chemical Industry,2011,19(3):13-15,30. |
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| Authors: | CHEN Lin-lin TANG Xiu-qin DENG Fan-zheng |
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| Affiliation: | CHEN Lin-lin,TANG Xiu-qin,DENG Fan-zheng(College of Chemistry and Materials Science,Huaibei Normal University,Huaibei 235000,China) |
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| Abstract: | The interaction between Eu3+ and BSA was investigated by fluorescence spectroscopy in ionic liquid aqueous two-phase.The study results indicated that Eu3+ has ability to quench the intrinsic fluorescence of BSA through a static quenching procedure.The fluorescence data were treated by using the double logarithmic equation,and the binding constants Ka of the interaction of Eu3+ with BSA and the number of binding sites n were obtained.The thermodynamic parameters of the interaction between Eu3+ and BSA at dif... |
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| Keywords: | Europium ion Bovine serum albumin(BSA) Fluorescence quenching Interaction Ionic liquid aqueous two-phase |
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