灵芝肽对乙醇诱导肝损伤小鼠的保护作用

目的：研究灵芝肽(GLP)对乙醇诱导的肝损伤小鼠的保护作用。方法：将60 只小鼠随机分成6 组，每组10 只，分别为正常组，乙醇肝损伤模型组，硫普罗宁(Tiopronin)阳性对照组，GLP 低、中和高剂量组。每天灌胃一次，GLP 低、中、高剂量组分别灌胃GLP 60、120、180mg/kg bw，阳性对照组灌胃硫普罗宁溶液50mg/kg bw，正常组、模型组灌胃等量生理盐水；每次给药3h 后，除正常组外，各组均灌胃50% 乙醇16mL/kg bw，连续灌胃2 周，建立小鼠酒精性肝损伤模型。通过脏器质量和体质量计算肝、脾脏指数；按照试剂盒的方法测定小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)含量，肝匀浆中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量、还原性谷胱甘肽(GSH)含量等指标。结果：60mg/kg bw GLP 能极显著降低酒精性肝损伤所引起的ALT、AST 活性和MDA、TG 含量的升高(P ＜ 0.01)，有效地拮抗肝脏中SOD的活性与GSH含量的降低(P ＜ 0.05～0.01)。结论：60mg/kg bw GLP 对乙醇诱导的肝损伤小鼠有显著的保护作用，180mg/kg bw GLP 的保肝效果好于50mg/kg bw硫普罗宁。

Hepatoprotective Effect of Ganoderma lucidum Peptides against Alcohol-induced Liver Injury in Mice

The hepatoprotective effect of Ganoderma lucidum peptides(GLP) against alcohol-induced liver injury in mice was investigated.Totally 60 mice were randomly divided into 6 groups of 10 mice each: normal group,alcohol model group,tioronin positive control group and GLP treatment groups.The mice in GLP groups were daily administered GLP via gavage tubes at doses of 60,120 mg/kg bw and 180 mg/kg bw,respectively.Positive control group was administered tioronin at the dose of 50 mg/kg bw and other groups were admi...