Preparative separation of four sesquiterpenoids from Curcuma longa by high-speed counter-current chromatography |
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Authors: | Yanqing Zhou Chunming Wang Ruibing Wang Ligen Lin Hao Hu |
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Affiliation: | State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China |
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Abstract: | A high-speed counter-current chromatography (HSCCC) method was developed for the preparative separation of four major sesquiterpenoids with similar structures, namely ar-turmerone, β-turmerone, α-turmerone, and E-α-atlantone, from the essential oil of Curcuma longa Linn. using a two-phase solvent system composed of n-heptane-ethyl acetate–acetonitrile–water (9.5/0.5/9/1, v/v). From a single HSCCC run, 1.7861 g of ar-turmerone, 0.4708 g of β-turmerone, 1.3427 g of α-turmerone, and 0.1650 g of E-α-atlantone were obtained. The purity of each compound was over 98% as determined by HPLC. The chemical structures of these compounds were determined by 1H NMR and 13C NMR. |
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Keywords: | Curcuma longa essential oil high-speed counter-current chromatography sesquiterpenoid turmerone |
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