The effect of radiopacifiers agents on pH,calcium release,radiopacity, and antimicrobial properties of different calcium hydroxide dressings |
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Authors: | Ronald Ordinola‐Zapata Clovis Monteiro Bramante Franklin GarcÍa‐godoy Bertram Ivan Moldauer Paloma Gagliardi Minotti Larissa TercíLIA Grizzo Marco Antonio Hungaro Duarte |
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Affiliation: | 1. Bauru Dental School, University of S?o Paulo, S?o Paulo, Brazil;2. Department of Operative Dentistry, Dental Materials and Endodontics, Dental School of Bauru, University of S?o Paulo, S?o Paulo, Brazil;3. Bioscience Research Center, College of Dentistry, University of Tennessee Health Science Center, Memphis, Tennessee;4. Larkin Community Hospital, Miami, Florida;5. Department of Biological Science, Bauru Dental School, University of S?o Paulo, S?o Paulo, Brazil |
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Abstract: | The aim of this study was to evaluate the antimicrobial activity, pH level, calcium ion release, and radiopacity of calcium hydroxide pastes associated with three radiopacifying agents (iodoform, zinc oxide, and barium sulfate). For the pH and calcium release tests, 45 acrylic teeth were utilized and immersed in ultrapure water. After 24 h, 72 h, and 7 days the solution was analyzed by using a pH meter and an atomic absorption spectrophotometer. Polyethylene tubes filled with the pastes were used to perform the radiopacity test. For the antimicrobial test, 25 dentin specimens were infected intraorally in order to induce the biofilm colonization and treated with the pastes for 7 days. The Live/Dead technique and a confocal microscope were used to obtain the ratio of live cells. Parametric and nonparametric statistical tests were performed to show differences among the groups (P < 0.05). The pH analysis at 7 days showed significant differences (P < 0.05) among the groups. No differences among the pastes were found in the calcium release test on the 7th day (P > 0.05). The calcium hydroxide/iodoform samples had the highest radiopacity and antimicrobial activity against the biofilm‐infected dentin in comparison to the other pastes (P < 0.05). Calcium hydroxide mixed with 17% iodoform and 35% propylene glycol into a paste had the highest pH, calcium ion release, radiopacity, and the greatest antimicrobial action versus similar samples mixed with BaSO4 or ZnO. Microsc. Res. Tech. 78:620–625, 2015. © 2015 Wiley Periodicals, Inc. |
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Keywords: | confocal microscopy biofilms dentistry calcium hydroxide |
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