Different bacterial models for in vitro induction of non‐cavitated enamel caries‐like lesions: Microhardness and polarized light miscroscopy analyses |
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Authors: | Priscila Hernández De Campos Mariane Emi Sanabe Jonas Almeida Rodrigues Danilo Antonio Duarte Maria Teresa Botti Rodrigues Santos Renata Oliveira Guaré cristiane Duque Adrian Lussi Michele Baffi Diniz |
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Affiliation: | 1. Department of Pediatric Dentistry, Cruzeiro Do Sul University—UNICSUL, S?o Paulo—SP, Brazil;2. Department of Pediatric Dentistry, Camilo Castelo Branco University—UNICATELO, S?o Paulo—SP, Brazil;3. Department of Pediatric Dentistry, School of Dentistry, Rio Grande Do Sul Federal University—UFRGS, Porto Alegre, RS, Brazil;4. Patients with Special Needs, Cruzeiro Do Sul University—UNICSUL, S?o Paulo—SP, Brazil;5. Department of Pediatric Dentistry, Ara?atuba School of Dentistry, S?o Paulo State University – UNESP, Ara?atuba, SP, Brazil;6. Department of Preventive, Restorative and Pediatric Dentistry, School of Dental Medicine, University of Bern—UNIBE, Bern, CH, Switzerland |
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Abstract: | The aim of this study was to compare different bacterial models for in vitro induction of non‐cavitated enamel caries‐like lesions by microhardness and polarized light microscopy analyses. One hundred blocks of bovine enamel were randomly divided into four groups (n = 25) according to the bacterial model for caries induction: (A) Streptococcus mutans, (B) S. mutans and Lactobacillus acidophilus, (C) S. mutans and L. casei, and (D) S. mutans, L. acidophilus, and L. casei. Within each group, the blocks were randomly divided into five subgroups according to the duration of the period of caries induction (4–20 days). The enamel blocks were immersed in cariogenic solution containing the microorganisms, which was changed every 48 h. Groups C and D presented lower surface hardness values (SMH) and higher area of hardness loss (ΔS) after the cariogenic challenge than groups A and B (P < 0.05). As regards lesion depth, under polarized light microscopy, group A presented significantly lower values, and groups C and D the highest values. Group B showed a higher value than group A (P < 0.05). Groups A and B exhibited subsurface caries lesions after all treatment durations, while groups C and D presented erosion‐type lesions with surface softening. The model using S. mutans, whether or not it was associated with L. acidophilus, was less aggressive and may be used for the induction of non‐cavitated enamel caries‐like lesions. The optimal period for inducing caries‐like lesions was 8 days. Microsc. Res. Tech. 78:444–451, 2015. © 2015 Wiley Periodicals, Inc. |
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Keywords: | tooth enamel tooth demineralization bacteria hardness tests microscopy |
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