Morphology of sealant/enamel interface after surface treatment with bioactive glass |
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Authors: | Fernanda de Carvalho Panzeri Pires‐de‐Souza Renata Espíndola Silveira Gabriel Abuna Michelle Alexandra Chinelatti Carla Cecilia Alandia‐Román Mario Alexandre Coelho Sinhoreti |
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Affiliation: | 1. Department of Dental Materials and Prosthodontics, Ribeir?o Preto School of Dentistry, University of S?o Paulo, Ribeir?o Preto, S?o Paulo, Brazil;2. Department of Restorative Dentistry, Piracicaba School of Dentistry, State University of Campinas, Piracicaba, S?o Paulo, Brazil |
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Abstract: | The purpose of this study was to analyze, by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), the morphology of sealant/enamel interface after surface treatment with Biosilicate. Before pits and fissures sealing, the occlusal surfaces of 10 sound human molars were sectioned perpendicularly at the fissures in order to obtain three slices for each tooth. Slices were randomly assigned into three groups (n = 10) according to sealing protocol: Group 1‐ Acid etching + Biosilicate + glass ionomer‐based sealant (Clinpro XT Varnish, 3M ESPE); Group 2‐ Acid etching + glass ionomer‐based sealant (Clinpro XT Varnish, 3M ESPE); Group 3‐ No sealing. All slices were subjected to thermal cycling (5,000 cycles; 5–55°C; dwell time: 30s). Half of the slices from each group (n = 5) were analyzed by CLSM and the other half by SEM. Groups 1 and 2 were also submitted to EDS analysis and their data were evaluated by Two‐Way ANOVA e Tukey's test (α=5%). EDS data analysis showed higher amounts of silicon (Si) ions than calcium (Ca) ions in Group 1 (P < 0.05); Group 2 presented higher amounts (P < 0.05) of Ca ions than Si ions. It may be concluded that the use of Biosilicate for surface treatment did not affect the morphology of glass ionomer‐based sealant/enamel interfaces. Microsc. Res. Tech. 78:1062–1068, 2015. © 2015 Wiley Periodicals, Inc. |
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Keywords: | dental materials bioactive glass dental sealants tooth remineralization |
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