An improved method for the scanning electron microscopy of spermatozoa

The authors carried out this research on an SEM technique and obtained a good preservation of spermatozoa. Among the many fixatires tested, potassium di-chromate mixed with osmium tetroxide and NaCl gave the best results. Generally, a rapid freeze drying of the fixed material, after a treatment with dimethylsul-phoxide, isopentane and liquid nitrogen improves the general standard of preservation.