好氧/延长闲置生物除磷系统中的聚磷菌特征

以往的研究发现,普通序批式活性污泥系统在适当延长闲置时间时可激发一些微生物产生过量摄磷的代谢响应。然而,此工艺中潜在聚磷微生物仍有待进一步识别。因此,本研究结合多种分子生物学手段对好氧/延长闲置生物除磷系统中的聚磷菌特征进行了系统的研究。研究表明,在以实际生活污水运行的好氧/延长闲置生物除磷系统中,传统的聚磷菌Accumulibacter仍然大量存在,其丰度为18%±4%,这说明传统的聚磷菌Accumulibacter可以在无厌氧段的条件下生存。另外,好氧/延长闲置生物除磷系统中,菌胶团类细菌Zooglea与伯克氏菌Burkholderiaceae也占有大量的比例,其丰度分别为27%±5%与17%±6%。同步FISH与聚磷染色进一步表明,菌胶团类细菌Zooglea可以在好氧条件下合成聚磷而伯克氏菌Burkholderiaceae没有明显的聚磷菌显型。研究证实了,除广泛接受的传统聚磷菌Accumulibacter外,好氧/延长闲置生物除磷系统存在着大量的其它聚磷微生物。

The Characteristics of Polyphosphate Accumulating Organisms Involved in Aerobic/Extended- Idle Biological Phosphorus Removal System

Previous studies showed that excess phosphorus uptake would be induced by some cells in a conventional activated sludge system if the idle period could be extended suitably. However, potential polyphosphate accumulationg organisms （PAOs） involved in the process needed to be further identified. The detailed characteristics of PAOs involved in the aerobic/extended -idle biological phosphorus removal systems were investigated. The results showed that the conventional PAOs （Accumulibacter） still presented in the aerobic/extended - idle process using real wastewater, with their abundance of 18% ＋ 4% , which indicated the conventional PAOs could thrive without a defined anaerobic zone. Besides, bacterial Zooglea and Burkholderiaceae were also found to be accounted for 27% _＋ 5% and 17% ＋ 6%, respectively. Further analysis combining FISH and polyphosphate staining revealed that bacterium related to Zoogloea could accumulate polyphosphate aerobically whereas bacterial Burkholderiaceae did not have the clear phenotype of PAOs. The results proved that apart from the widely accepted Accumulibacter, other PAOs substantially presented in the aerobic/ extended - idle process.