Surface versus bulk activity of lysozyme deposited on hydrogel contact lens materials in vitro

Purpose

To determine and compare the levels of surface versus bulk active lysozyme deposited on several commercially available hydrogel contact lens materials.

Methods

Hydrogel contact lens materials [polymacon, omafilcon A, nelfilcon A, nesofilcon A, ocufilcon and etafilcon A with polyvinylpyrrolidone (PVP)] were incubated in an artificial tear solution for 16?h. Total activity was determined using a standard turbidity assay. The surface activity of the deposited lysozyme was determined using a modified turbidity assay. The amount of active lysozyme present within the bulk of the lens material was calculated by determining the difference between the total and surface active lysozyme.

Results

The etafilcon A materials showed the highest amount of total lysozyme activity (519?±?8?μg/lens, average of Moist and Define), followed by the ocufilcon material (200?±?5?μg/lens) and these two were significantly different from each other (p?

Conclusions

The surface and bulk activity of lysozyme deposited on contact lenses is material dependent. Lysozyme deposited on ionic, high water content lens materials such as etafilcon A show significantly higher surface and bulk activity than many other hydrogel lens materials.