| 黄伞菌丝体多糖提取工艺及免疫调节作用研究 |
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| 引用本文： | 宫春宇,胡清秀,韩永超,崔鹏举,王霄婷. 黄伞菌丝体多糖提取工艺及免疫调节作用研究[J]. 食品工业, 2012, 0(8): 48-51 |
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| 作者姓名： | 宫春宇 胡清秀 韩永超 崔鹏举 王霄婷 |
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| 作者单位： | 齐齐哈尔大学食品与生物工程学院;黑龙江省普通高校农产品加工重点实验室;中国农业科学院农业资源与农业区划研究所 |
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| 基金项目： | 齐齐哈尔大学青年教师科研启动支持计划-科技重点,项目编号2011k-Z09 |
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| 摘 要： | 在单因素试验基础上运用正交试验方法确定了黄伞菌丝体多糖最佳提取工艺,即菌丝体粉碎粒径为80目、加水量1∶15、提取时间2.5 h、提取温度80℃、提取1次,醇析多糖时使用无水乙醇、3.5倍于多糖溶液、醇析12 h;同时研究了黄伞菌丝体多糖在体外刺激小鼠脾淋巴细胞增殖作用,结果表明黄伞菌丝体多糖可以促进淋巴细胞增殖,具有体外调节免疫作用。
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| 关 键 词： | 黄伞 多糖 提取工艺 菌丝体 免疫活性 |
Study on Extracting Process of Myclia Amylose from Pholiota.adi posa and Their Immunocompetence |
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| Gong Chun-yu,Hu Qing-xiu,Han Yong-chao,Cui Peng-ju,Wang Xiao-ting. Study on Extracting Process of Myclia Amylose from Pholiota.adi posa and Their Immunocompetence[J]. The Food Industry, 2012, 0(8): 48-51 |
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| Authors: | Gong Chun-yu Hu Qing-xiu Han Yong-chao Cui Peng-ju Wang Xiao-ting |
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| Affiliation: | 1.College of Food and Bioengineering,Qiqihar University(Qiqihar 161006);2.Heilongjiang Provincial Key University Laboratory of Processing Agricultural Products(Qiqihar 161006);3.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agriculture Science(Beijing 100081) |
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| Abstract: | By using the method of single factor analysis and orthogonal experimental design,the condition was studied in which the myclia amylose of Pholiota.adi posa could be extracted the best.The results indicated that the best conditions were the ratio between powder and water 1:15,the extraction time 2.5 h,the extraction temperature 80 ℃,and extracting for 1 times.When precipitating the amylose by ethanol,the conditions were the density of ethanol 100%,the volume ratio between extraction water and ethanol 1:3.5,and precipitating for 12 h.Meanwhile,stimulating the proliferation of mouse spleen lymphocytes by amylose from Pholiota.adi posa myclia was studied,with result showing that they could bring the proliferation of lymphocytes and enhancement of immunity. |
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| Keywords: | Pholiota.adi posa amylose extraction processing myclia immunocompetence |
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