盐藻油微波辅助提取工艺优化

对盐藻油微波辅助提取工艺进行优化，采用响应面法分析液料比、浸提温度和浸提时间对盐藻油得率的影响，建立相应的预测模型。方差分析结果表明，液料比(P＜0.005)、浸提温度(P＜0.005)和浸提时间(P＜0.005)都对盐藻油得率有显著影响，液料比的二次项(P＜0.005)和浸提温度的二次项(P＜0.005)对盐藻油得率有显著影响，并且液料比和浸提温度间存在交互作用(P＜0.025)。最佳工艺条件为液料比10:1(mL/g)、浸提温度54℃、浸提时间9.7min，在该条件下，盐藻油得率(15.79±0.97)%。微波辅助提取的盐藻油中主要含有棕榈酸、油酸、亚油酸和亚麻酸，质量分数分别为13.85%、5.30%、8.60%、23.75%。

Optimization of Microwave-assisted Extraction Technology for Oil from Dunaliella salina

Microwave-assisted extraction technology for oil from Dunaliella salina was investigated.Response surface methodology was used to optimize the extraction parameters on the basis of the extraction rate of the oil.Three independent factors such as material-liquid ratio,extraction time and extraction temperature were evaluated.The optimal extraction conditions were material-liquid ratio of 1:10(g/mL),extraction temperature of 54 ℃ and extraction time of 9.7 min.Under the optimal extraction conditions,the extraction rate of oil from Dunaliella salina was(15.79 ± 0.97)%.The major components in Dunaliella salina oil were palmitic acid,oleic acid,linoleic acid and linolenic acid with the contents of 13.85%,5.30%,8.60% and 23.75%,respectively.