大孔树脂法纯化玉竹总皂苷的工艺研究

目的：筛选出分离、纯化玉竹总皂苷的大孔树脂型号及工艺条件。方法：通过静态吸附与解吸实验确定大孔树脂的型号。在静态吸附与解吸单因素试验基础上，利用四因素四水平正交试验对LSA-33型大孔树脂纯化玉竹总皂苷的工艺进行研究，以总皂苷吸附率、解吸率为指标，确定最佳工艺。结果：LSA-33型树脂对玉竹总皂苷具有较好的纯化效果，其最佳工艺为：pH6～7的玉竹提取液，树脂吸附4h，解吸液为体积分数95%的乙醇，解吸液用量与树脂质量比37.5:1(mL/g)，解吸时间为2h时，玉竹浸膏中总皂苷的含量由未纯化前的12.28%提高到41.75%。结论：LSA-33型大孔树脂可较好地纯化玉竹总皂苷，且操作简单、安全、成本低廉，有较高的应用价值。

Purification of Total Saponins from Rhizoma Polygonati Odorati by Macroporous Resin Adsorption

Objectives: To find out the best type of macroporous resin and determine the optimal process conditions for separation and purification of total saponins from Rhizoma Polygonati Odorati. Methods: The best type of macroporous adsorption resin for total saponins was determined by static adsorption and desorption experiments. Based on one-factor-at-a-time experiments, a four-factor, three-level orthogonal array design was employed for the optimization of four process parameters for improved adsorption and desorption rates. Results: LSA-33 macroporous resin was found to be the best resin among 6 types of macroporous resin tested. The best purification conditions for total saponins were determined as follows: crude extract from Rhizoma Polygonati Odorati (pH 6－7) was adsorbed for 4 h and then desorbed with 95% ethanol at a solvent-to-solid ratio of 37.5:1 (mL/g) for 2 h. As a result, the content of total saponins in the crude extract was increased to 41.75% from 12.28%. Conclusion: LSA-33 macroporous resin is effective in purifying total saponins from Rhizoma Polygonati Odorati with the advantages of easy operation, high safety and low cost and thus will have high application value.