Systematic Comparison of Plant Promoters in Nicotiana spp. Expression Systems |
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| Authors: | Ekaterina S. Shakhova Nadezhda M. Markina Tatiana Mitiouchkina Evgenia N. Bugaeva Tatiana A. Karataeva Kseniia A. Palkina Liliia I. Fakhranurova Ilia V. Yampolsky Karen S. Sarkisyan Alexander S. Mishin |
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| Affiliation: | 1.Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia;2.Department of Translational Medicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia;3.Synthetic Biology Group, MRC London Institute of Medical Sciences, London W12 0NN, UK;4.Institute of Clinical Sciences, Faculty of Medicine and Imperial College Centre for Synthetic Biology, Imperial College London, London W12 0NN, UK |
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| Abstract: | We report a systematic comparison of 19 plant promoters and 20 promoter-terminator combinations in two expression systems: agroinfiltration in Nicotiana benthamiana leaves, and Nicotiana tabacum BY-2 plant cell packs. The set of promoters tested comprised those not present in previously published work, including several computationally predicted synthetic promoters validated here for the first time. The expression of EGFP driven by different promoters varied by more than two orders of magnitude and was largely consistent between two tested Nicotiana systems. We confirmed previous reports of significant modulation of expression by terminators, as well as synergistic effects of promoters and terminators. Additionally, we observed non-linear effects of gene dosage on expression level. The dataset presented here can inform the design of genetic constructs for plant engineering and transient expression assays. |
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