Nitro Dihydrocapsaicin,a Non-Pungent Capsaicin Analogue,Inhibits Cellular Senescence of Lens Epithelial Cells via Upregulation of SIRT1 |
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| Authors: | Pussadee Paensuwan Thanet Laorob Jatuporn Ngoenkam Uthai Wichai Sutatip Pongcharoen |
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| Affiliation: | 1.Department of Optometry, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand;2.Department of Chemistry, Faculty of Science, Naresuan University, Phitsanulok 65000, Thailand;3.Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand;4.Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok 65000, Thailand |
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| Abstract: | Diabetic cataracts are a common complication that can cause blindness among patients with diabetes mellitus. A novel nitro dihydrocapsaicin (NDHC), a capsaicin analog, was constructed to have a non-pungency effect. The objective of this research was to study the effect of NDHC on human lens epithelial (HLE) cells that lost function from hyperglycemia. HLE cells were pretreated with NDHC before an exposure to high glucose (HG) conditions. The results show that NDHC promoted a deacceleration of cellular senescence in HLE cells. This inhibition of cellular senescence was characterized by a delayed cell growth and lower production of reactive oxygen species (ROS) as well as decreased SA-β-galactosidase activity. Additionally, the expression of Sirt1 protein sharply increased, while the expression of p21 and phospho-p38 proteins decreased. These findings provide evidence that NDHC could exert a pharmacologically protective effect by inhibiting the senescence program of lens cells during diabetic cataracts. |
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| Keywords: | lens epithelial cell diabetic cataract senescence oxidative stress SIRT1 |
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