储藏稻谷优势霉菌的分离鉴定及特征分析

以湖南长沙和湖北襄阳仓储稻谷为对象,对稻谷储藏期间主要优势霉菌进行分离纯化,记录其在高盐察氏培养基上的菌落形态。通过蔡司显微镜分别拍摄了主要优势霉菌的显微结构特征,并通过主要优势霉菌的DNA提取和测序,在基因水平上对霉菌种类进行了鉴定。经鉴定,主要优势霉菌分别为:黄曲霉、黑曲霉、白曲霉、灰绿曲霉和产黄青霉。

Separation, identification and characteristics analysis of dominant mould in stored rice

The rice stored in Changsha (in Hunan province) and Xiangyang (in Hubei Province) was chosen as the object to separate the dominant mold from the stored rice and purify them, and record the colony morphology in Salt Czapek Dox Agar. The microstructure characteristics of the main dominant mold were photographed by Zeiss microscope respectively. The main dominant mold were identified at the genetic level by extracting and sequencing the DNA, which were aspergillus flavus, aspergillus niger, aspergillus albicans, aspergillus glaucus and penicillium chrysogenum.