基于点击化学的壳聚糖水凝胶的制备及性能

本研究首先在甲磺酸中用丙烯酰氯对壳聚糖(CS)进行接枝改性,通过一步法合成了水溶性丙烯酰基壳聚糖(CS-AC).之后,在光引发剂I2959和紫外光(UV)照下,以二硫苏糖醇(DTT)为交联剂制备了基于巯基-烯点击化学的CS-AC/DTT快速交联水凝胶.红外光谱(FTIR)和核磁共振氢谱(1 H-NMR)结果定性和定量地...

Preparation and Properties of Chitosan Hydrogels Based on Click Chemistry

A water soluble acryloyl chitosan(CS-AC)was synthesized through modifying chitosan(CS)with acryloyl chloride in methylsulfonic acid.Under UV exposure,a fast crosslinking CS-AC/DTT hydrogel was then prepared based on thiol-ene click chemistry using dithithitol(DTT)as crosslinker and I2959 as photoinitiator.Infrared spectroscopy(FTIR)and nuclear magnetic resonance(¹H-NMR)results qualitatively and quantitatively proved the vinyl grafting rate of around 100%.Comparing with the gelation time over 40 min of self-crosslinking hydrogel CS-CC-CS,that of click chemistry hydrogel CS-AC/DTT was shortened within 10 min with a vinyl/thiol feed ratio of 1∶0.5.Characterization results demonstrated the gel strength of 5-10 KPa and excellent swelling rate up to 1415%.Furthermore,cytotoxicity and antibacterial tests of hydrogels exhibited excellent cytocompatibility towards NIH-3 T3 cells and antibacterial properties towards resistant staplylococcus aureus methicillin-resistant staplylococcus aureus(MRSA).The above results reveal the potential application in drug loading and antibiosis for the synthesized hydrogel materials.