| 产CLA嗜酸乳杆菌的筛选诱变与鉴定 |
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| 引用本文： | 李志刚,翟爱华,张东杰,李志江.产CLA嗜酸乳杆菌的筛选诱变与鉴定[J].中国酿造,2012,31(6):69-73. |
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| 作者姓名： | 李志刚 翟爱华 张东杰 李志江 |
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| 作者单位： | 黑龙江八一农垦大学食品学院,黑龙江大庆,163319 |
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| 基金项目： | 黑龙江省教育厅科技攻关项目（115211980） |
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| 摘 要： | 从传统泡菜中选育高产共轭亚油酸(Conjugated Linoleic Acid,CLA)的菌株L28,利用人工诱变方法,以L28为出发菌株,采用紫外线、亚硝基胍(NTG)依次诱变及紫外、亚硝基胍复合诱变处理,经进一步液体发酵复筛获得多株突变菌株,CLA的最优产量为95.1425μg/mL.其中L44突变菌株为CLA生成能力最高菌株.经16S rDNA全序列分析法和生理生化试验确定菌株的种属,然后经过Gene Bank基因比对确定同源基因,最后经鉴定为嗜酸乳杆菌(Lactobacillus acidophilus).
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| 关 键 词： | 共轭亚油酸 嗜酸乳杆菌 鉴定 诱变 |
Screening, breeding and identification of conjugated linoleic acid-producing Lactobacillus acidophilus by mutation |
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| LI Zhigang
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ZHAI Aihua
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ZHANG Dongjie
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LI Zhijiang.Screening, breeding and identification of conjugated linoleic acid-producing Lactobacillus acidophilus by mutation[J].China Brewing,2012,31(6):69-73. |
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| Authors: | LI Zhigang ZHAI Aihua ZHANG Dongjie LI Zhijiang |
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| Affiliation: | (College of Food, Heilongjiang Bayi Agricultural University, Daqing 163319, China) |
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| Abstract: | Alactic acid bacterial strain, L28, having the ability to synthesize conjugated linoleic acid （CLA） was isolated from pickle juice samples. analysis and API system.The L28 as first strain was treated by ultraviolet and NTG to obtain several mutant strains, which obtain 95.1425μg/ml for CLA production. The strain was named L44. The strain L44 through physiological and biochemical identification combining and the full-length 16S rDNA sequence analysis was Lactobacillus acidophilus. |
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| Keywords: | conjugated linoleic acid（CLA） Lactobacillus acidophilus identification mutation |
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