| 中高温大曲中产酯酶细菌的筛选 |
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| 引用本文： | 刘阳,薛正莲,黄祖耀,赵梦梦.中高温大曲中产酯酶细菌的筛选[J].中国酿造,2012,31(8):99-102. |
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| 作者姓名： | 刘阳 薛正莲 黄祖耀 赵梦梦 |
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| 作者单位： | 安徽工程大学生物与化学工程学院,微生物发酵安徽省工程技术研究中心,安徽芜湖241000 |
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| 摘 要： | 通过在培养基中添加底物三丁酸甘油酯,从中、高温大曲中初步筛选到产酯酶细菌15株.以乙酸对硝基苯酯为底物,其中12#菌株表现出最高催化活力其酶活为0.83U/mL;以乙醇、己酸为底物,其中10#菌株表现出最高催化活力其酶活为6.7U/mL.对上述两种测定方法中相对酶活较高的菌株恒温振荡培养(36℃、150r/min) 72h后取其发酵液离心(12000r/min,4℃)20min进行气相色谱分析,结果表明这些菌株都能产生一定量的酸类、醇类和酯类物质.
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| 关 键 词： | 酯酶 中温大曲 高温大曲 细菌 |
Screening of esterase-producing bacteria from medium and high temperature Daqu |
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| LIU Yang
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XUE Zhenglian
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HUANG Zuyao
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ZHAO Mengmeng.Screening of esterase-producing bacteria from medium and high temperature Daqu[J].China Brewing,2012,31(8):99-102. |
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| Authors: | LIU Yang XUE Zhenglian HUANG Zuyao ZHAO Mengmeng |
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| Affiliation: | (Institute of Biologic ＆ Chemical Engineering, Anhui Polytechnic Universi(y, Microorganism Fermentation Engineering and Technology Research Center of Anhui Province, Wuhu 241000, China) |
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| Abstract: | Through adding glyeeryl tri-n-butyrate in the culture me dium, 15 strains producing esterase are obtained after the primary screening in medium and high temperature Daqu. Then using 4-nitrophenyl acetate as substrate, the strain 12# shows the highest catalytic activity of 0.83U/ml; using ethanol and caproic acid as substrate, the strain 10# shows the highest catalytic activity of 33.5U/ml. The two selected strains with relatively higher esterase activity are cultured for 72h at 36℃ with shaking by 150r/min, and the fermented supernatants were centrifuged in 12000r/min at 4℃ for 20min and analysed by GC. The results show that these strains can produce certain amount of acids, alcohol and esters. |
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| Keywords: | esterase medium temperature Daqu high temperature Daqu bacteria |
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