| 反相高效液相色谱法测定婴儿奶粉中乳铁蛋白的含量 |
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| 引用本文： | 王飞,窦文渊,石燕丽,农云军,苏流坤. 反相高效液相色谱法测定婴儿奶粉中乳铁蛋白的含量[J]. 中国酿造, 2012, 31(7): 167-168 |
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| 作者姓名： | 王飞 窦文渊 石燕丽 农云军 苏流坤 |
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| 作者单位： | 中国广州分析测试中心广东省化学危害应急检测技术重点实验室,广东广州,510070 |
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| 基金项目： | 广东省自然科学基金资助项目（7300055）;中国广州分析测试中心自筹资金科研项目（20100008） |
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| 摘 要： | 建立了反相高效液相色谱法(RP-HPLC)测定婴儿奶粉中乳铁蛋白的含量,采用C4色谱柱洗脱,二极管阵列检测器(波长220nm)测定.流动相A为0.1％三氟乙酸水溶液,流动相B为乙腈-水-三氟乙酸(体积比90∶10∶0.1).线性范围为5mg/L～1000mg/L,相关系数为0.9999,定量下限为30mg/kg,加标回收率为86.0％～91.7％.该方法用于实际样品的测定,样品处理简单,结果令人满意.
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| 关 键 词： | 反相高效液相色谱 婴儿奶粉 乳铁蛋白 |
Determination of lactoferrin in infant formula powder milk by reversed phase high performance liquid chromatography |
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| WANG Fei , DOU Wenyuan , SHI Yanli , NONG Yunjun , SU Liukun. Determination of lactoferrin in infant formula powder milk by reversed phase high performance liquid chromatography[J]. China Brewing, 2012, 31(7): 167-168 |
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| Authors: | WANG Fei DOU Wenyuan SHI Yanli NONG Yunjun SU Liukun |
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| Affiliation: | (Guangdong Key Laboratory of Emergency Chemical Test,China National Analytical Center,Guangzhou 510070,China) |
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| Abstract: | A method for the determination of lactoferrin in infant formula powder milk by RP-HPLC was developed.Lactoferrin in infant formula powder milk was successfully separated on a C4 column by a gradient elution using 0.1% trifluoroacetic acid as mobile phase A and acetonitrile-water-trifluoroacetic acid（90∶10∶0.1,by volume） as mobile phase B.The linear detection range was 5mg/L～1000mg/L（r=0.9999） and the limit of quantity（LOQ） was 30mg/kg.The recovery was in the range of 86.0%～91.7%.The results of the actual sample indicated the method was simple accurate and reproducible. |
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| Keywords: | RP-HPLC； infant formula powder milk； lactoferrin |
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